Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021, Zarzouna, Tunisia.
Environ Sci Pollut Res Int. 2014 Mar;21(6):4461-72. doi: 10.1007/s11356-013-2404-4. Epub 2013 Dec 12.
The effects of permethrin (PER) on a panel of antoxidant enzymes; superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and indices of protein oxidation status (carbonylation and free thiols) were determined in digestive gland and gills of the clam Ruditapes decussatus. Animals were exposed to 100 ppb PER for 2 days. These enzyme activities increased significantly in digestive gland (p<0.05) after PER treatment and oxidative modification of proteins was detected in both gill and digestive gland extracts using redox proteomics. PER exposure significantly reduced the amount of protein free thiol groups in digestive gland rather than in gill, when compared to controls. Conversely, digestive gland showed significantly higher levels of carbonylated proteins than gill after PER exposure. Some proteins were successfully identified by mass spectrometry of tryptic peptides. Our data suggest that digestive gland of R. decussatus can be used as a model tissue for investigating environmental risk of PER contamination.
研究了林丹（PER）对贻贝（Ruditapes decussatus）消化腺和鳃中抗氧化酶（超氧化物歧化酶[SOD]、过氧化氢酶[CAT]和谷胱甘肽 S-转移酶[GST]）和蛋白质氧化状态指标（羰基化和游离巯基）的影响。将动物暴露于 100ppb PER 中 2 天。暴露于 PER 后，贻贝消化腺中的这些酶活性显著增加（p<0.05），并使用氧化还原蛋白质组学在贻贝和消化腺提取物中检测到蛋白质的氧化修饰。与对照组相比，PER 暴露后，与 gill 相比，digestive gland 中蛋白质游离巯基的含量显著降低。相反，暴露于 PER 后，digestive gland 中的羰基化蛋白质水平明显高于 gill。通过胰蛋白酶肽的质谱分析成功鉴定了一些蛋白质。我们的数据表明，R. decussatus 的消化腺可作为研究 PER 污染环境风险的模型组织。
