Melchior F, Kindl H
University of Marburg, Department of Chemistry, FRG.
FEBS Lett. 1990 Jul 30;268(1):17-20. doi: 10.1016/0014-5793(90)80961-h.
Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full-length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into the vectors pKK233-2 and pDS12/RBSII-2, respectively. Expression of the cDNA in Escherichia coli yielded an enzymatically active dimer exhibiting solely stilbene synthase activity. The protein was characterized by enzyme activity and Western blot analysis.
芪合酶负责白藜芦醇及其他芪类化合物的形成，这些芪类化合物在葡萄中作为植保素发挥作用。从葡萄信使核糖核酸（mRNA）中制备了全长芪合酶互补脱氧核糖核酸（cDNA）并进行了测序。将编码含392个氨基酸多肽的pSV25中的插入片段分别插入载体pKK233-2和pDS12/RBSII-2中。该cDNA在大肠杆菌中的表达产生了一种仅表现出芪合酶活性的具有酶活性的二聚体。通过酶活性和蛋白质免疫印迹分析对该蛋白质进行了表征。
