Schwekendiek A, Pfeffer G, Kindl H
University of Marburg, Department of Chemistry, Germany.
FEBS Lett. 1992 Apr 13;301(1):41-4. doi: 10.1016/0014-5793(92)80206-v.
Stilbene synthase cDNAs were isolated from a pine (Pinus sylvestris) cDNA library. Poly(A)+RNA required for the preparation was obtained from young seedlings challenged with Botrytis cinerea. A full-length cDNA encoding pinosylvin-forming stilbene synthase was sequenced, and the deduced amino acid sequence was compared with sequences of resveratrol-forming stilbene synthases. The cDNA coding for the key enzyme of pinosylvin formation is a valuable tool for detecting early effects of environmental stress in pine needles.
从松树（欧洲赤松）cDNA文库中分离出芪合酶cDNA。制备所需的聚腺苷酸加尾RNA是从受灰葡萄孢菌侵染的幼苗中获得的。对编码生成松白藜的芪合酶的全长cDNA进行了测序，并将推导的氨基酸序列与生成白藜的芪合酶序列进行了比较。编码松白藜形成关键酶的cDNA是检测松针环境胁迫早期效应的宝贵工具。
