Kuhnt U, Kelly M J, Schaumberg R
Exp Brain Res. 1979 Apr 2;35(2):371-85. doi: 10.1007/BF00236621.
Intracellular recordings were performed in slices of the lateral geniculate body, the visual cortex, and the hippocampus of guinea pigs. After recording, Procion Yellow was iontophoretically injected. During the injection period the neurones were activated antidromically and/or transynaptically. Up to 6 h after dye injection slices were fixed in a buffered formaldehyde/sucrose solution and later histologically processed. In the lateral geniculate body, after orthodromic stimulation across the optic tract, optic tract fibers were stained secondarily. Orthodromic activation across intrageniculate connections led to secondarily stained geniculate neurones. In the visual cortex orthodromic activation of the injected neurones across white matter led to secondarily stained fibres travelling in the direction to the white matter or to secondarily stained neurones in the vicinity of the injected neurone. In the hippocampus anti- and orthodromic activation across the alveus or the mossy fibre pathway led to a secondary staining of neighbouring pyramidal neurones and to a secondary staining of fibres which where afferent to the injected neurone. All slices where Procion Yellow was detected in the extracellular space were rejected. These findings led to the conclusion that Procion Yellow must have been transported across chemical synapses from the post- to the presynaptic side. The transport occurred only if the concerned synapses were activated.
在豚鼠的外侧膝状体、视觉皮层和海马体切片上进行细胞内记录。记录后，通过离子电泳注入普施安黄。在注入期间，神经元被逆向和/或经突触激活。在染料注入后长达6小时内，将切片固定在缓冲甲醛/蔗糖溶液中，随后进行组织学处理。在外侧膝状体中，通过视束进行顺向刺激后，视束纤维会被二次染色。通过膝状体内连接进行顺向激活会导致膝状体神经元被二次染色。在视觉皮层中，通过白质对注入神经元进行顺向激活会导致向白质方向行进的纤维被二次染色，或者在注入神经元附近的神经元被二次染色。在海马体中，通过海马槽或苔藓纤维通路进行逆向和顺向激活会导致相邻锥体细胞被二次染色，以及注入神经元的传入纤维被二次染色。所有在细胞外空间检测到普施安黄的切片都被排除。这些发现得出的结论是，普施安黄必定是从突触后侧向突触前侧跨化学突触转运的。只有在相关突触被激活时才会发生这种转运。
