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携带卵清蛋白CD4+和CD8+ T细胞表位的重组流感病毒的免疫原性

Immunogenicity of a recombinant influenza virus bearing both the CD4+ and CD8+ T cell epitopes of ovalbumin.

作者信息

Garulli Bruno, Di Mario Giuseppina, Sciaraffia Ester, Kawaoka Yoshihiro, Castrucci Maria R

机构信息

Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy.

出版信息

J Biomed Biotechnol. 2011;2011:497364. doi: 10.1155/2011/497364. Epub 2011 Oct 5.

DOI:10.1155/2011/497364
PMID:22007143
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3189626/
Abstract

Recombinant influenza viruses that bear the single immunodominant CD8+ T cell epitope OVA(257-264) or the CD4+ T cell epitope OVA₃₂₃₋₃₃₉ of the model antigen ovalbumin (OVA) have been useful tools in immunology. Here, we generated a recombinant influenza virus, WSN-OVA(I/II), that bears both OVA-specific CD8+ and CD4+ epitopes on its hemagglutinin molecule. Live and heat-inactivated WSN-OVA(I/II) viruses were efficiently presented by dendritic cells in vitro to OT-I TCR transgenic CD8+ T cells and OT-II TCR transgenic CD4+ T cells. In vivo, WSN-OVA(I/II) virus was attenuated in virulence, highly immunogenic, and protected mice from B16-OVA tumor challenge in a prophylactic model of vaccination. Thus, WSN-OVA(I/II) virus represents an additional tool, along with OVA TCR transgenic mice, for further studies on T cell responses and may be of value in vaccine design.

摘要

携带模型抗原卵清蛋白（OVA）的单一免疫显性CD8⁺T细胞表位OVA(257 - 264)或CD4⁺T细胞表位OVA₃₂₃₋₃₃₉的重组流感病毒，已成为免疫学中的有用工具。在此，我们构建了一种重组流感病毒WSN - OVA(I/II)，其血凝素分子上同时带有OVA特异性CD8⁺和CD4⁺表位。活的和热灭活的WSN - OVA(I/II)病毒在体外能被树突状细胞有效地呈递给OT - I TCR转基因CD8⁺T细胞和OT - II TCR转基因CD4⁺T细胞。在体内，WSN - OVA(I/II)病毒毒力减弱、免疫原性高，并且在预防性疫苗接种模型中能保护小鼠免受B16 - OVA肿瘤攻击。因此，WSN - OVA(I/II)病毒与OVA TCR转基因小鼠一样，是用于进一步研究T细胞反应的额外工具，并且可能在疫苗设计中有价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/ca1c7eb46718/JBB2011-497364.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/397fe4d5f86f/JBB2011-497364.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/f6a82bc46dc0/JBB2011-497364.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/021515f4cd80/JBB2011-497364.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/38dfbd3321e4/JBB2011-497364.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/ca1c7eb46718/JBB2011-497364.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/397fe4d5f86f/JBB2011-497364.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/f6a82bc46dc0/JBB2011-497364.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/021515f4cd80/JBB2011-497364.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/38dfbd3321e4/JBB2011-497364.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e25/3189626/ca1c7eb46718/JBB2011-497364.005.jpg

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