Xu Hu, Wang Mei, Liu Xun-Hong, Xu Jian-Ya, Fu Xing-Sheng, Zhou Yi-Zhi
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Yao Xue Xue Bao. 2011 Aug;46(8):1004-7.
In this study, laser scanning confocal microscopy (LSCM) was used to determine the location and relative quantity of flavonoids in the leaves of Apocynum venetum L. from the top, middle and basal parts of the branch. The leaves of the plants of one, two and three years old, separately, were collected in July. ANOVA and LSD test were employed in the statistical analysis. The results indicated that flavonoids located mainly in xylem conduit of vein, collenchyma, epidermic cells and cuticle. The data of flavonoids contents under statistical analysis showed that difference existed in the leaves of different parts and different ages. This study provided the reliable scientific material about the analysis of the ecological and the exploitation of the leaves of Apocynum venetom L.
在本研究中，利用激光扫描共聚焦显微镜（LSCM）来确定罗布麻枝条顶部、中部和基部叶片中黄酮类化合物的位置和相对含量。分别于7月采集一年生、二年生和三年生植株的叶片。统计分析采用方差分析和最小显著差数法检验。结果表明，黄酮类化合物主要位于叶脉的木质部导管、厚角组织、表皮细胞和角质层中。经统计分析的黄酮类化合物含量数据表明，不同部位和不同年龄的叶片存在差异。本研究为罗布麻叶片的生态分析和开发利用提供了可靠的科学资料。
