Tan Zhijian, Yi Yongjian, Wang Hongying, Zhou Wanlai, Wang Chaoyun
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Molecules. 2016 Mar 4;21(3):262. doi: 10.3390/molecules21030262.
Ionic liquids (ILs) are considered as green solvents, and widely applied for the extraction of various compounds.
The present research focuses on the extraction of flavonoids from Apocynum venetum L. leaves by ultrasound-assisted extraction (UAE). Several major influencing factors were optimized. Then, an aqueous biphasic system (ABS) was applied for further isolation of flavonoids.
The flavonoids were mainly distributed in the top phase, while impurities were extracted to the bottom phase. The parameters influencing the extraction, namely type and concentration of salt, temperature, and pH, were studied in detail. Under optimized conditions (72.43% IL extract, 28.57% (NH4)2SO4, 25 °C temperature, pH 4.5), the preconcentration factor and extraction efficiency were found to be 3.78% and 93.35%, respectively.
This simple and efficient methodology is expected to see great use in the extraction and isolation of pharmaceutically active components from medicinal plant resources.
离子液体（ILs）被视为绿色溶剂，并广泛应用于各种化合物的萃取。
本研究聚焦于通过超声辅助萃取（UAE）从罗布麻叶中提取黄酮类化合物。对几个主要影响因素进行了优化。然后，应用双水相体系（ABS）进一步分离黄酮类化合物。
黄酮类化合物主要分布在上层，而杂质被萃取到下层。详细研究了影响萃取的参数，即盐的类型和浓度、温度及pH值。在优化条件下（72.43%离子液体萃取物、28.57%硫酸铵、25℃温度、pH值4.5），预富集因子和萃取效率分别为3.78%和93.35%。
这种简单高效的方法有望在从药用植物资源中提取和分离药用活性成分方面得到广泛应用。
