Barts Cancer Institute, Queen Mary University of London, Centre for Tumour Biology, London, UK.
J Dermatolog Treat. 2013 Jun;24(3):209-14. doi: 10.3109/09546634.2011.631978. Epub 2011 Nov 10.
The nucleoside analogue 4-thiothymidine has shown great potential in vitro as a photosensitiser for the photodynamic therapy of numerous cancer cell lines. However, the limited penetrating power of UV-A radiation, to which it responds, raises doubts as to its practical usefulness in clinical applications. We addressed this issue by studying the penetration extent of topical thiothymidine and the antiproliferative effect of its combination with UV-A radiation on ex vivo basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) skin cancer biopsies, and normal skin. Our results show that both the intralesional concentration of the drug and the intensity of UV-A radiation are sufficient to activate the molecule and cause extensive death by apoptosis of the malignant cells. Normal skin biopsies were not significantly affected by the treatment.
核苷类似物 4-硫代胸腺嘧啶在体外作为众多癌细胞系光动力疗法的光敏剂显示出巨大的潜力。然而，它所响应的 UV-A 辐射的穿透能力有限，这使得人们对其在临床应用中的实际用途产生了怀疑。我们通过研究局部硫代胸腺嘧啶的穿透程度以及其与 UV-A 辐射联合应用对体外基底细胞癌（BCC）和鳞状细胞癌（SCC）皮肤癌活检和正常皮肤的抗增殖作用来解决这个问题。我们的结果表明，药物的瘤内浓度和 UV-A 辐射的强度足以激活分子并导致恶性细胞广泛凋亡死亡。正常皮肤活检不受治疗的显著影响。
