Department of Pediatrics, Chinese PLA General Hospital, Beijing 100853, China.
J Pediatr Surg. 2011 Oct;46(10):1948-55. doi: 10.1016/j.jpedsurg.2011.06.024.
Spontaneous regression is usually found in stage 4s neuroblastoma, whereas the elucidation of the underlying molecular mechanism(s) is still limited.
Our study aims to investigate the pathogenesis of spontaneous regression at the protein level.
Differential expression of proteins in stage 4s neuroblastoma tissue, in stage 4 neuroblastoma tissue, and in normal adrenal tissue was investigated by use of 2-dimensional difference gel electrophoresis (2D-DIGE).
Twenty-four protein spots were found to have significant changes among the different tissues, in which 16 proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Among these proteins, 7 proteins (RhoGDP-dissociation inhibitor 1, phosphatidylethanolamine-binding protein, prohibitin, etc) were up-regulated and 2 proteins (F-actin capping protein 1 subunit and aldose reductase) were down-regulated in stage 4s neuroblastoma compared with stage 4 neuroblastoma. The differential expression of selected candidate protein (RhoGDP-dissociation inhibitor 1 and CAPZA1) was further validated by western blotting.
Some proteins are differentially expressed between stage 4s and stage 4 neuroblastoma tissue, including those associated with differentiation and proliferation as well as apoptosis. RhoGDP-dissociation inhibitor 1 is highly expressed in stage 4s neuroblastoma tissue, whereas CAPZA1 is down-regulated.
自发消退通常发生在 4S 期神经母细胞瘤中，而阐明其潜在的分子机制仍然有限。
本研究旨在从蛋白质水平研究自发消退的发病机制。
通过二维差异凝胶电泳（2D-DIGE）研究 4S 期神经母细胞瘤组织、4 期神经母细胞瘤组织和正常肾上腺组织中的蛋白质差异表达。
在不同组织中发现 24 个蛋白点具有显著变化，其中 16 个蛋白使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）鉴定。在这些蛋白质中，与 4 期神经母细胞瘤相比，7 种蛋白质（RhoGDP 解离抑制剂 1、磷脂酰乙醇胺结合蛋白、抑制素等）上调，2 种蛋白质（F-肌动蛋白加帽蛋白 1 亚基和醛糖还原酶）下调。通过 Western 印迹进一步验证了选定候选蛋白（RhoGDP 解离抑制剂 1 和 CAPZA1）的差异表达。
4S 期和 4 期神经母细胞瘤组织之间存在一些差异表达的蛋白质，包括与分化、增殖和凋亡相关的蛋白质。RhoGDP 解离抑制剂 1 在 4S 期神经母细胞瘤组织中高表达，而 CAPZA1 下调。
