Kalkus J, Reh M, Schlegel H G
Institut für Mikrobiologie, Georg-August-Universität, Göttingen, Federal Republic of Germany.
J Gen Microbiol. 1990 Jun;136(6):1145-51. doi: 10.1099/00221287-136-6-1145.
Several linear megaplasmids were detected in the facultatively lithoautotrophic Gram-positive bacterium Nocardia opaca. The wild-type strain MR11 contains, in addition to the cccDNA plasmids pHG31-a and pHG31-b, the linear plasmids pHG201 (270 kb), pHG202 (400 kb) and pHG203 (420 kb). The wild-type strain MR22 contains, in addition to the cccDNA plasmid pHG33, the linear plasmids pHG204 (180 kb), pHG205 (280 kb) and pHG206 (510 kb). After preparation of DNA from cells embedded in agarose, the linear plasmids were demonstrated by pulsed-field electrophoresis. By means of DNA probes for genes of soluble hydrogenase and ribulose-bisphosphate carboxylase, the conjugative plasmids pHG201 and pHG205 were shown to be the carriers of the genetic information for these enzymes. A restriction map of pHG201 for the enzymes AsnI, SpeI, XbaI is presented.
在兼性化能自养革兰氏阳性细菌不透明诺卡氏菌中检测到了几种线性大质粒。野生型菌株MR11除了含有共价闭合环状DNA（cccDNA）质粒pHG31-a和pHG31-b外，还含有线性质粒pHG201（270 kb）、pHG202（400 kb）和pHG203（420 kb）。野生型菌株MR22除了含有cccDNA质粒pHG33外，还含有线性质粒pHG204（180 kb）、pHG205（280 kb）和pHG206（510 kb）。从包埋在琼脂糖中的细胞制备DNA后，通过脉冲场电泳证实了线性质粒的存在。通过针对可溶性氢化酶和核酮糖-1,5-二磷酸羧化酶基因的DNA探针，表明接合性质粒pHG201和pHG205是这些酶的遗传信息载体。给出了pHG201针对AsnI、SpeI、XbaI酶的限制性图谱。
