Okata Yuichi, Hisamatsu Chieko, Nishijima Eiji, Okita Yutaka
Division of Pediatric Surgery, Department of Surgery, Kobe University Graduate School of Medicine, 7-5-2, Kusunoki-cho, Chuo-ku, Kobe, 650-0017, Japan.
Pediatr Surg Int. 2012 Jan;28(1):43-9. doi: 10.1007/s00383-011-3007-0.
To investigate the effect of topical application of basic fibroblast growth factor (bFGF) after caustic esophageal injury in rats.
Thirty-six male rats were randomly divided into three groups. Corrosive esophageal injury was produced by internal application of 30% sodium hydroxide (NaOH) solution to the distal esophagus. Group A rats were uninjured. Group B rats were injured and untreated. Group C rats were injured and received topical bFGF (10 μg/ml). Surviving rats were killed at 28 days. The survival rate, body weight gain, symptoms and histopathological changes that included tissue damage score, ratio of esophageal luminal area/total esophageal area (LA/TA) and the proportion of a neural marker PGP 9.5-positive area were assessed.
The survival rate and the prevalence of symptoms were not significantly different between Groups B and C. Although the tissue damage score did not differ in Groups B and C, LA/TA was significantly higher in Group C than in Group B. The proportion of the PGP 9.5-positive area was significantly lower in Groups B and C than in Group A; however, it was higher in Group C than in Group B.
Topical application of bFGF was effective in preventing stricture after NaOH-induced esophagitis.
研究碱性成纤维细胞生长因子（bFGF）局部应用于大鼠腐蚀性食管损伤后的效果。
36只雄性大鼠随机分为三组。通过向食管远端内注入30%氢氧化钠（NaOH）溶液造成腐蚀性食管损伤。A组大鼠未受伤。B组大鼠受伤但未治疗。C组大鼠受伤并接受局部bFGF（10μg/ml）治疗。存活的大鼠在28天时处死。评估存活率、体重增加情况、症状以及组织病理学变化，包括组织损伤评分、食管腔面积/食管总面积比值（LA/TA）和神经标志物PGP 9.5阳性区域的比例。
B组和C组的存活率和症状发生率无显著差异。虽然B组和C组的组织损伤评分无差异，但C组的LA/TA显著高于B组。B组和C组的PGP 9.5阳性区域比例显著低于A组；然而，C组高于B组。
局部应用bFGF对预防NaOH诱导的食管炎后的狭窄有效。
