Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil.
Cell Tissue Res. 2011 Nov;346(2):237-44. doi: 10.1007/s00441-011-1239-x. Epub 2011 Oct 19.
Lectins constitute a class of glycoproteins, which are capable of selectively and reversibly binding to carbohydrates, distinguishing small structural differences in complex oligosaccharides. Studies have shown that the binding of lectins to cell-surface carbohydrates can lead to various effects such as cellular proliferation, histamine release and cytokine production. Canavalia brasiliensis lectin (ConBr) is a (D-mannose) D-glucose lectin. In this study, murine splenocytes were cultured to determine the effect of ConBr on cell proliferation, nitric oxide (NO) release and cytokine secretion. In addition, cellular viability assays were performed to evaluate any mitogenic activity induced by this lectin. ConBr significantly increased cell proliferation with minimal cell damage. This lectin was able to induce an increased production of cytokines such as IL-2, IL-6 and IFN-γ and a decreased production of IL- 10. The release of NO was also observed. The results of this study indicate that ConBr could potentially be used as an immunomodulator.
凝集素是一类糖蛋白，能够选择性和可逆地与碳水化合物结合，区分复杂寡糖中的微小结构差异。研究表明，凝集素与细胞表面碳水化合物的结合会导致各种效应，如细胞增殖、组胺释放和细胞因子产生。巴西栗凝集素（ConBr）是一种（D-甘露糖）D-葡萄糖凝集素。在这项研究中，培养了鼠脾细胞以确定 ConBr 对细胞增殖、一氧化氮（NO）释放和细胞因子分泌的影响。此外，还进行了细胞活力测定，以评估该凝集素诱导的任何有丝分裂活性。ConBr 可显著增加细胞增殖，而对细胞损伤最小。这种凝集素能够诱导细胞因子如 IL-2、IL-6 和 IFN-γ的产生增加，同时降低 IL-10 的产生。还观察到 NO 的释放。本研究结果表明，ConBr 可能可用作免疫调节剂。
