巴西刀豆凝集素对B16F10细胞的抗增殖作用。
Antiproliferative effect of Canavalia brasiliensis lectin on B16F10 cells.
作者信息
Silva Flávio de Oliveira, Santos Priscila das Neves, Figueirôa Evellyne de Oliveira, de Melo Cristiane Moutinho Lagos, de Andrade Lemoine Neves Juliana Kelle, Arruda Francisco Vassiliepe Sousa, Cajazeiras João Batista, do Nascimento Kyria Santiago, Teixeira Edson Holanda, Cavada Benildo Sousa, Porto Ana Lúcia Figueiredo, Pereira Valéria Rêgo Alves
机构信息
Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Pernambuco, Brazil.
Centro de Ciências biológicas, Universidade Federal de Pernambuco, Pernambuco, Brazil.
出版信息
Res Vet Sci. 2014 Apr;96(2):276-82. doi: 10.1016/j.rvsc.2014.01.005. Epub 2014 Feb 11.
Lectins are proteins or glycoproteins with the ability to link to carbohydrates at the cell surface in a specific and reversible manner. Studies have showed that lectins are demonstrate immunomodulatory and antitumor agents. This study aims to evaluate the effect of lectin extracted from the seeds of Canavalia brasiliensis (ConBr) on murine melanoma B16F10 cells by analyzing cell viability, apoptosis index, cell migration, production of cytokines and nitric oxide (NO). Results showed that ConBr was able to reduce cell viability and thwart apoptosis, which could be observed by decrease in cell migration. ConBr also induced NO and IL-12 synthesis. Altogether, these data demonstrate the potential of ConBr as a therapeutic agent for melanoma.
凝集素是一类蛋白质或糖蛋白，能够以特异性且可逆的方式与细胞表面的碳水化合物结合。研究表明，凝集素是具有免疫调节和抗肿瘤作用的物质。本研究旨在通过分析细胞活力、凋亡指数、细胞迁移、细胞因子和一氧化氮（NO）的产生，评估从巴西刀豆种子中提取的凝集素（ConBr）对小鼠黑色素瘤B16F10细胞的影响。结果显示，ConBr能够降低细胞活力并抑制凋亡，这可通过细胞迁移的减少来观察到。ConBr还诱导了NO和IL-12的合成。总之，这些数据证明了ConBr作为黑色素瘤治疗药物的潜力。
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