Müller T
Institute for Anatomy, University of Tübingen, F.R.G.
J Neurosci Methods. 1990 Jun;32(3):223-6. doi: 10.1016/0165-0270(90)90144-5.
Saturated methylene blue solutions or powder were applied with a brush to fresh, unfixed brain slabs. The specimens were then exposed to ambient oxygen in a moist chamber. Fixation was done by application of an ammonium heptamolybdate solution followed by a phosphate-buffered paraformaldehyde/glutaraldehyde mixture with phosphomolybdic acid added. The specimens were dehydrated in tert-butanol before embedding in paraffin. Because of its high specificity for perikarya and for varicose, unmyelinated nerve fibres, this technique is a helpful supplement to other histological methods, particularly to myelin staining techniques. It is therefore well-suited for routine use in neuroanatomical research.
用刷子将饱和亚甲蓝溶液或粉末涂抹在新鲜的、未固定的脑切片上。然后将标本置于潮湿小室中暴露于环境氧气中。通过施加七钼酸铵溶液，接着是添加了磷钼酸的磷酸盐缓冲多聚甲醛/戊二醛混合物进行固定。标本在叔丁醇中脱水后再嵌入石蜡。由于该技术对神经细胞体以及曲张的无髓神经纤维具有高度特异性，它是其他组织学方法，特别是髓鞘染色技术的有益补充。因此，它非常适合在神经解剖学研究中常规使用。
