Müller T
Institute for Anatomy, University of Tübingen, Federal Republic of Germany.
Stain Technol. 1989 Mar;64(2):93-6. doi: 10.3109/10520298909108052.
Two steps are recommended for fixation of central nervous tissue after supravital staining with methylene blue: application of ammonium heptamolybdate followed by a paraformaldehyde-glutaraldehyde mixture. The two fixatives complement each other so that both dye and tissue are optimally fixed. Molybdate renders the dye water and alcohol insoluble, the aldehyde mixture conditions the tissue for embedding in paraffin. This technique is a suitable alternative to conventional whole mount and frozen sectioning methods.
在用亚甲蓝进行超活染色后，固定中枢神经组织推荐采取两个步骤：先使用钼酸铵，然后使用多聚甲醛 - 戊二醛混合物。这两种固定剂相互补充，从而使染料和组织都得到最佳固定。钼酸盐使染料不溶于水和酒精，醛混合物使组织适合石蜡包埋。该技术是传统整装和冷冻切片方法的合适替代方案。
