Müller T
Z Mikrosk Anat Forsch. 1989;103(6):967-70.
The described method for selective staining of neurons in the central nervous system of vertebrates and invertebrates involves the application of minute crystals of methylene blue or small amounts of dye solution to the tissue. Staining, however, depends on the diffusion qualities of the dye and not on active axonal transport mechanisms. Furthermore, the production of air-dried frozen sections after fixation by ammonium heptamolybdate is considered the best procedure for exact assessment of the results. This method is well-suited for routine use in both neuroanatomic and electrophysiologic research.
所描述的用于对脊椎动物和无脊椎动物中枢神经系统中的神经元进行选择性染色的方法，包括将亚甲蓝微晶或少量染料溶液应用于组织。然而，染色取决于染料的扩散特性，而非活跃的轴突运输机制。此外，用七钼酸铵固定后制作空气干燥的冷冻切片，被认为是精确评估结果的最佳步骤。该方法非常适合在神经解剖学和电生理学研究中常规使用。
