Center for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, Research Organization of Information and Systems, Yata, Mishima 411-8540, Japan.
Nucleic Acids Res. 2012 Jan;40(Database issue):D54-6. doi: 10.1093/nar/gkr854. Epub 2011 Oct 18.
New generation sequencing platforms are producing data with significantly higher throughput and lower cost. A portion of this capacity is devoted to individual and community scientific projects. As these projects reach publication, raw sequencing datasets are submitted into the primary next-generation sequence data archive, the Sequence Read Archive (SRA). Archiving experimental data is the key to the progress of reproducible science. The SRA was established as a public repository for next-generation sequence data as a part of the International Nucleotide Sequence Database Collaboration (INSDC). INSDC is composed of the National Center for Biotechnology Information (NCBI), the European Bioinformatics Institute (EBI) and the DNA Data Bank of Japan (DDBJ). The SRA is accessible at www.ncbi.nlm.nih.gov/sra from NCBI, at www.ebi.ac.uk/ena from EBI and at trace.ddbj.nig.ac.jp from DDBJ. In this article, we present the content and structure of the SRA and report on updated metadata structures, submission file formats and supported sequencing platforms. We also briefly outline our various responses to the challenge of explosive data growth.
新一代测序平台正在产生具有更高通量和更低成本的数据。这些能力的一部分用于个人和社区的科学项目。随着这些项目发表，原始测序数据集被提交到主要的下一代序列数据存档中，即序列读取档案（SRA）。存档实验数据是可重复科学进展的关键。SRA 是作为国际核苷酸序列数据库合作组织（INSDC）的一部分，为下一代序列数据建立的公共存储库。INSDC 由美国国立生物技术信息中心（NCBI）、欧洲生物信息学研究所（EBI）和日本 DNA 数据库（DDBJ）组成。SRA 可从 NCBI 上的 www.ncbi.nlm.nih.gov/sra、EBI 上的 www.ebi.ac.uk/ena 和 DDBJ 上的 trace.ddbj.nig.ac.jp 访问。在本文中，我们介绍了 SRA 的内容和结构，并报告了更新的元数据结构、提交文件格式和支持的测序平台。我们还简要概述了我们应对数据爆炸式增长挑战的各种措施。
