Department of Poultry Science, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
Poult Sci. 2011 Nov;90(11):2656-62. doi: 10.3382/ps.2011-01342.
Clostridium perfringens is recognized as an enteric pathogen in humans, domestic animals, and livestock. This organism is associated with necrotic enteritis, gangrenous dermatitis, clostridial dermatitis (turkeys), and gizzard erosions in poultry. This study was conducted to evaluate the effectiveness of a direct-fed microbial (DFM), Primalac (Star Labs, Clarksdale, MO), in preventing intestinal colonization of turkey poults with C. perfringens. One-day-old turkey poults (n = 128) were randomly divided into 4 treatments with 4 replicates (8 birds/pen). Treatments were as follows: 1) basal diet without DFM (C); 2) basal diet supplemented with Primalac (1.5 kg/ton; PM); 3) basal diet with poults gavaged with C. perfringens (CCP); and 4) basal diet supplemented with Primalac and poults gavaged with C. perfringens (PMCP). Feed and water were provided ad libitum throughout the trials, and birds were inoculated with C. perfringens (10(8)cfu/mL) on d 3 and 7. On d 21, 2 birds/pen were killed, spleen and bursa of Fabricius were collected and weighed, and cecal contents were used for C. perfringens enumeration. Feed consumption, BW, and feed conversion were calculated throughout the trial (weekly and cumulatively). Data were analyzed using GLM of SAS (SAS Institute, Cary, NC; P < 0.05). Among the inoculated groups, birds fed the DFM-supplemented diet had significantly lower cecal C. perfringens counts than the birds fed the diet without the DFM. The C. perfringens (log(10) cfu/g) in ceca were as follows: C, 5.88; CCP, 7.26; PM, 5.35; PMCP, 6.19 ± 0.36. No differences were observed for BW (814 ± 11 g), feed conversion (1.33 ± 0.03), organ weights, or relative organ weights. Further studies are needed to fully ascertain the potential of using DFM to reduce the numbers of C. perfringens in the gastrointestinal tract of turkey poults.
产气荚膜梭菌被认为是人类、家畜和牲畜的肠道病原体。该病原体与坏死性肠炎、坏疽性皮炎、梭状芽胞杆菌性皮炎（火鸡）和家禽的肌胃侵蚀有关。本研究旨在评估直接饲喂微生物（DFM）Primalac（Star Labs，Clarksdale，MO）预防火鸡雏肠道定植产气荚膜梭菌的效果。将 1 日龄火鸡雏（n = 128）随机分为 4 个处理组，每组 4 个重复（8 只/栏）。处理如下：1）不含 DFM 的基础日粮（C）；2）基础日粮添加 Primalac（1.5 千克/吨；PM）；3）基础日粮灌喂产气荚膜梭菌（CCP）；4）基础日粮添加 Primalac 并灌喂产气荚膜梭菌（PMCP）。试验期间自由采食和饮水，雏鸡于第 3 天和第 7 天灌喂产气荚膜梭菌（10(8)cfu/mL）。第 21 天，每栏取 2 只鸡，采集脾脏和法氏囊称重，并取盲肠内容物进行产气荚膜梭菌计数。试验期间（每周和累计）计算采食量、BW 和饲料转化率。数据采用 SAS 的 GLM 进行分析（SAS Institute，Cary，NC；P < 0.05）。在接种组中，饲喂添加 DFM 的日粮的鸡盲肠中产气荚膜梭菌数量明显低于未添加 DFM 的日粮。盲肠中产气荚膜梭菌（log(10)cfu/g）如下：C 组为 5.88；CCP 组为 7.26；PM 组为 5.35；PMCP 组为 6.19 ± 0.36。BW（814 ± 11 g）、饲料转化率（1.33 ± 0.03）、器官重量或相对器官重量均无差异。需要进一步研究以充分确定使用 DFM 减少火鸡雏胃肠道中产气荚膜梭菌数量的潜力。
