Biagini Lucia, Galosi Livio, Tambella Adolfo Maria, Roncarati Alessandra, De Bellis Danilo, Pesaro Stefano, Attili Anna-Rita, Berardi Sara, Rossi Giacomo
School of Biosciences and Veterinary Medicine, University of Camerino, 62024 Matelica, Italy.
Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, 33100 Udine, Italy.
Animals (Basel). 2024 Nov 27;14(23):3435. doi: 10.3390/ani14233435.
The interest for in ovo feeding has grown in the last decades mainly concerning probiotics, live microorganisms that can actively interact with the embryo. The aim of this study was to evaluate the effects of a multi-strain probiotic diluted in Marek's disease vaccine (MDV) on zootechnical performances, intestinal morphology and spp. infection. One hundred and twenty eggs of Ross 308 broiler chickens were incubated until 18 d, when 105 fertilised and vital eggs were randomly allocated into three groups. A control group (C) was inoculated with MDV; two treated groups (P1 and P2) were inoculated with MDV and different concentrations of probiotics: 1 × 10 CFU/100 μL in P1 and 1 × 10 CFU/100 μL in P2. After hatching, chickens were separated into three replicates (10/replicate). Zootechnical parameters were determined. At the end of the cycle (35 d), chickens were slaughtered, and the intestine was collected for morphological analysis from nine chickens per group (three/replicate). spp. oocyst shedding was determined weekly, and parasitic lesions were analysed on the histological sample. In ovo treatment with probiotic did not influence hatching rate but significantly improved body weight and positively influenced intestinal morphometric data compared to C. Oocyst shedding in faeces resulted in an increase in C, with significant differences at sampling performed at 14, 21 and 28 d of age. These results suggest that the tested probiotic compound is safe for in ovo supplementation and effectively improves zootechnical performances and coccidian resistance.
在过去几十年中，人们对卵内投喂的兴趣有所增加，主要涉及益生菌，即能够与胚胎积极相互作用的活微生物。本研究的目的是评估稀释在马立克氏病疫苗（MDV）中的多菌株益生菌对畜牧生产性能、肠道形态和球虫感染的影响。将120枚罗斯308肉鸡种蛋孵化至18日龄，此时将105枚受精且有活力的种蛋随机分为三组。对照组（C）接种MDV；两个处理组（P1和P2）接种MDV和不同浓度的益生菌：P1组为1×10CFU/100μL，P2组为1×10CFU/100μL。孵化后，将雏鸡分成三个重复组（每组10只）。测定畜牧生产性能参数。在试验周期结束时（35日龄），宰杀雏鸡，每组选取9只雏鸡（每个重复组3只）采集肠道进行形态学分析。每周测定球虫卵囊排出情况，并对组织学样本进行寄生虫损伤分析。与对照组相比，卵内使用益生菌处理不影响孵化率，但显著提高了体重，并对肠道形态学数据产生了积极影响。粪便中的卵囊排出量在对照组中增加，在14、21和28日龄时采样有显著差异。这些结果表明，所测试的益生菌化合物用于卵内补充是安全的，并能有效提高畜牧生产性能和抗球虫能力。
