Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.
Nat Prod Res. 2012 Nov;26(21):2020-3. doi: 10.1080/14786419.2011.628670. Epub 2011 Oct 20.
Three naphthoquinones, plumbagin (1), 3,3'-biplumbagin (2) and elliptinone (3), isolated from Plumbago indica roots by antibacterial bioassay-guided isolation, were used as standard markers for quantitative determination. A reversed-phase HPLC method was established for the simultaneous determination of the naphthoquinones in P. indica root extracts. The method utilised a Phenomenex® ODS column (4.6 × 150 mm, 5 µm) at 25°C with a mixture of methanol and 5% aqueous acetic acid (80 : 20 v/v) as the mobile phase at a flow rate of 0.85 mL/min, and UV detection at 260 nm. The parameters of linearity, precision, accuracy specificity and sensitivity of the method were evaluated. The recovery of the method was 98.6-100.6% with good linearity (r (2 )≥ 0.9997) for all three naphthoquinones. A high degree of sensitivity, specificity as well as repeatability and reproducibility (R.S.D. values less than 5%) were also achieved.
三种萘醌类化合物，包括从印度麻根中通过抗菌生物测定法指导分离得到的白花丹素（1）、3,3'-双白花丹素（2）和椭圆酮（3），被用作定量测定的标准标记物。建立了一种反相高效液相色谱法，用于同时测定印度麻根提取物中的萘醌类化合物。该方法在 25°C 下使用 Phenomenex®ODS 柱（4.6×150mm，5μm），以甲醇和 5%的水醋酸（80：20v/v）混合物作为流动相，流速为 0.85mL/min，在 260nm 处进行紫外检测。评估了该方法的线性、精密度、准确度、特异性和灵敏度参数。该方法的回收率为 98.6-100.6%，对于所有三种萘醌类化合物均具有良好的线性（r²≥0.9997）。该方法还具有高灵敏度、特异性以及良好的重复性和重现性（R.S.D.值小于 5%）。
