Department of Seafood Processing Technology, Faculty of Fisheries, University of Cukurova, 01330 Balcali, Adana, Turkey.
Int J Food Sci Nutr. 2012 May;63(3):326-31. doi: 10.3109/09637486.2011.627844. Epub 2011 Oct 19.
This study aims to evaluate the efficiency of trans-methylation methods in fish oil obtained using Soxhlet extraction method and Bligh and Dyer method and also to observe the effects of extraction methods on the contents of polyunsaturated fatty acids [PUFAs, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)] in five marine species: sardine (Sardinella aurita), anchovy (Engraulis encrasicolus), sea bream (Sparus aurata), brushtooth lizardfish (Saurida undosquamis) and chub mackerel (Scomber japonicus). The results showed that Bligh and Dyer extraction method was more efficient in extracting polar and non-polar lipids than Soxhlet method and also prevented losses of PUFAs by a reduction in the oxidation. The level of EPA showed fluctuations for the two extraction methods. However, Soxhlet method showed significant decrease (p < 0.05) in the level of DHA for all fish species. The use of n-heptane proves to be superior for the recovery of unsaturated fatty acids, especially EPA and DHA.
本研究旨在评估索氏提取法和布莱迪-戴尔法提取鱼油中甲基转移效率，并观察不同提取方法对 5 种海洋鱼类（沙丁鱼、凤尾鱼、真鲷、狼牙虾虎鱼和竹荚鱼）多不饱和脂肪酸（尤其是二十碳五烯酸（EPA）和二十二碳六烯酸（DHA））含量的影响。结果表明，与索氏提取法相比，布莱迪-戴尔提取法更能有效地提取极性和非极性脂质，同时通过减少氧化来防止多不饱和脂肪酸的损失。两种提取方法的 EPA 水平均有波动。然而，索氏提取法使所有鱼类的 DHA 水平显著降低（p<0.05）。正己烷的使用证明对不饱和脂肪酸，特别是 EPA 和 DHA 的回收更优越。
