使用传统生物分析设备在液体处理分液吸头中实现干血斑提取自动化。
Use of conventional bioanalytical devices to automate DBS extractions in liquid-handling dispensing tips.
作者信息
Johnson Casey Jl, Christianson Chad D, Sheaff Chrystal N, Laine Derek F, Zimmer Jennifer Sd, Needham Shane R
机构信息
Alturas Analytics, Inc., 1324 Alturas Drive, Moscow, ID 83843, USA.
出版信息
Bioanalysis. 2011 Oct;3(20):2303-10. doi: 10.4155/bio.11.212.
BACKGROUND
Conventional liquid-handling devices were employed, along with an improved punching device, to semi-automate dried blood spot (DBS) extraction of alprazolam, α-hydroxyalprazolam and midazolam from human whole blood. Liquid-handling devices were used to add internal standard to the DBS cards and to extract the analytes from the DBS, in order to be analyzed by HPLC-MS/MS.
RESULTS
The technique was shown to be accurate (±12.0%) and precise (10.3%) across the dynamic range of the assay.
CONCLUSION
The semi-automated extraction reduced sample preparation time by more than 50% when compared with more conventional DBS manual extraction methods.
背景
使用传统的液体处理设备以及改进的打孔设备，对人全血中的阿普唑仑、α-羟基阿普唑仑和咪达唑仑进行干血斑（DBS）提取的半自动化操作。液体处理设备用于向DBS卡中添加内标，并从DBS中提取分析物，以便通过高效液相色谱-串联质谱（HPLC-MS/MS）进行分析。
结果
该技术在整个分析动态范围内显示出准确（±12.0%）和精密（10.3%）。
结论
与更传统的DBS手动提取方法相比，半自动化提取将样品制备时间减少了50%以上。
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