采用临床和实验室标准协会 M27-A3 肉汤微量稀释参考方法，通过与 48 小时测定比较，验证 24 小时氟胞嘧啶 MIC 测定的有效性。
Validation of 24-hour flucytosine MIC determination by comparison with 48-hour determination by the Clinical and Laboratory Standards Institute M27-A3 broth microdilution reference method.
机构信息
Mycotic Diseases Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mailstop G-11, Atlanta, GA 30333, USA.
出版信息
J Clin Microbiol. 2011 Dec;49(12):4322-5. doi: 10.1128/JCM.05479-11. Epub 2011 Oct 19.
Abstract
Flucytosine and itraconazole are the only antifungal agents for which the Clinical Laboratory and Standards Institute recommendations include MIC breakpoint readings at 48 h only. Here we show good essential and categorical agreement between the flucytosine MIC readings at 48 and 24 h for Candida species.
摘要
氟胞嘧啶和伊曲康唑是仅有的两种抗真菌药物，临床实验室标准化研究所的建议只包括在 48 小时时的 MIC 折点读数。在这里，我们显示了在 48 小时和 24 小时时氟胞嘧啶 MIC 读数之间对念珠菌属的良好的基本和分类一致性。
相似文献
1
Validation of 24-hour flucytosine MIC determination by comparison with 48-hour determination by the Clinical and Laboratory Standards Institute M27-A3 broth microdilution reference method.采用临床和实验室标准协会 M27-A3 肉汤微量稀释参考方法，通过与 48 小时测定比较，验证 24 小时氟胞嘧啶 MIC 测定的有效性。
J Clin Microbiol. 2011 Dec;49(12):4322-5. doi: 10.1128/JCM.05479-11. Epub 2011 Oct 19.
2
Comparison of the Sensititre YeastOne colorimetric microdilution panel and the NCCLS broth microdilution method for antifungal susceptibility testing against Candida species.用于念珠菌属抗真菌药敏试验的Sensititre YeastOne比色微量稀释板与NCCLS肉汤微量稀释法的比较。
Chemotherapy. 2002 Mar;48(1):21-5. doi: 10.1159/000048583.
3
4
Multicenter comparison of the sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory standards M27-A reference method for testing clinical isolates of common and emerging Candida spp., Cryptococcus spp., and other yeasts and yeast-like organisms.Sensititre YeastOne比色法抗真菌药敏板与美国国家临床实验室标准委员会M27-A参考方法对常见和新出现的念珠菌属、隐球菌属以及其他酵母菌和酵母样生物体临床分离株检测的多中心比较。
J Clin Microbiol. 1999 Mar;37(3):591-5. doi: 10.1128/JCM.37.3.591-595.1999.
5
Comparison of a spectrophotometric microdilution method with RPMI-2% glucose with the National Committee for Clinical Laboratory Standards reference macrodilution method M27-P for in vitro susceptibility testing of amphotericin B, flucytosine, and fluconazole against Candida albicans.用分光光度法微稀释法（RPMI-2%葡萄糖培养基）与美国国家临床实验室标准委员会参考宏稀释法M27-P，对两性霉素B、氟胞嘧啶和氟康唑针对白色念珠菌进行体外药敏试验的比较。
Antimicrob Agents Chemother. 1996 Sep;40(9):1998-2003. doi: 10.1128/AAC.40.9.1998.
6
Comparative evaluation of Etest and sensititre yeastone panels against the Clinical and Laboratory Standards Institute M27-A2 reference broth microdilution method for testing Candida susceptibility to seven antifungal agents.采用临床和实验室标准协会M27 - A2参考肉汤微量稀释法，对Etest和sensititre酵母样菌药敏板检测念珠菌对七种抗真菌药物敏感性进行比较评估。
J Clin Microbiol. 2007 Mar;45(3):698-706. doi: 10.1128/JCM.01840-06. Epub 2007 Jan 3.
7
Comparison of visual 24-hour and spectrophotometric 48-hour MICs to CLSI reference microdilution MICs of fluconazole, itraconazole, posaconazole, and voriconazole for Candida spp.: a collaborative study.针对念珠菌属，将氟康唑、伊曲康唑、泊沙康唑和伏立康唑的视觉24小时和分光光度法48小时最低抑菌浓度（MIC）与CLSI参考微量稀释法MIC进行比较：一项合作研究。
J Clin Microbiol. 2005 Sep;43(9):4535-40. doi: 10.1128/JCM.43.9.4535-4540.2005.
8
Multicenter comparison of the VITEK 2 antifungal susceptibility test with the CLSI broth microdilution reference method for testing amphotericin B, flucytosine, and voriconazole against Candida spp.VITEK 2抗真菌药敏试验与CLSI肉汤微量稀释参考方法针对两性霉素B、氟胞嘧啶和伏立康唑对念珠菌属进行检测的多中心比较
J Clin Microbiol. 2007 Nov;45(11):3522-8. doi: 10.1128/JCM.00403-07. Epub 2007 Oct 3.
9
Comparative evaluation of macrodilution and alamar colorimetric microdilution broth methods for antifungal susceptibility testing of yeast isolates.用于酵母分离株抗真菌药敏试验的常量稀释法与alamar比色微量稀释肉汤法的比较评估
J Clin Microbiol. 1995 Oct;33(10):2660-4. doi: 10.1128/jcm.33.10.2660-2664.1995.
10
Multi-centre evaluation of the Etest method for antifungal drug susceptibility testing of Candida spp. and Cryptococcus neoformans. BSAC Working Party on Antifungal Chemotherapy.念珠菌属和新型隐球菌抗真菌药敏试验的Etest法多中心评估。英国抗菌化疗学会工作组
J Antimicrob Chemother. 1998 Sep;42(3):321-31. doi: 10.1093/jac/42.3.321.
引用本文的文献
1
Variations in virulence factors, antifungal susceptibility and extracellular polymeric substance compositions of cryptic and uncommon Candida species from oral candidiasis.口腔念珠菌病中隐匿和罕见念珠菌物种的毒力因子、抗真菌药敏性及胞外聚合物组成的变异
BMC Oral Health. 2025 Jul 2;25(1):1029. doi: 10.1186/s12903-025-06334-2.
2
Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands.荷兰出现对氟胞嘧啶耐药的热带假丝酵母菌进化枝。
Emerg Infect Dis. 2025 Jul;31(7):1354-1364. doi: 10.3201/eid3107.241918.
3
Again and Again-Survival of in Urine Containing Antifungals.反复出现——在含有抗真菌药物的尿液中的生存情况。
Pharmaceutics. 2024 Apr 29;16(5):605. doi: 10.3390/pharmaceutics16050605.
4
Antifungal Effects and Potential Mechanisms of Benserazide Hydrochloride Alone and in Combination with Fluconazole Against .盐酸苄丝肼单独及与氟康唑联合抗. 的抗真菌作用及潜在机制。
Drug Des Devel Ther. 2021 Nov 16;15:4701-4711. doi: 10.2147/DDDT.S336667. eCollection 2021.
5
Development of Amphotericin B Micellar Formulations Based on Copolymers of Poly(ethylene glycol) and Poly(ε-caprolactone) Conjugated with Retinol.基于与视黄醇共轭的聚乙二醇和聚（ε-己内酯）共聚物的两性霉素B胶束制剂的研发
Pharmaceutics. 2020 Feb 25;12(3):196. doi: 10.3390/pharmaceutics12030196.
6
Identification of New Antifungal Agents Targeting Chitin Synthesis by a Chemical-Genetic Method.通过化学遗传学方法鉴定靶向几丁质合成的新型抗真菌剂。
Molecules. 2019 Aug 29;24(17):3155. doi: 10.3390/molecules24173155.
7
Live Monitoring and Analysis of Fungal Growth, Viability, and Mycelial Morphology Using the IncuCyte NeuroTrack Processing Module.使用 IncuCyte NeuroTrack 处理模块实时监测和分析真菌生长、活力和菌丝形态。
mBio. 2019 May 28;10(3):e00673-19. doi: 10.1128/mBio.00673-19.
8
Research of Mrr1, Cap1 and MDR1 in resistant to azole medications.Mrr1、Cap1和MDR1在抗唑类药物方面的研究。
Exp Ther Med. 2018 Feb;15(2):1217-1224. doi: 10.3892/etm.2017.5518. Epub 2017 Nov 16.
9
Synergistic Antifungal Effect of Fluconazole Combined with Licofelone against Resistant .氟康唑与利考昔芬联合使用对耐药菌的协同抗真菌作用
Front Microbiol. 2017 Nov 7;8:2101. doi: 10.3389/fmicb.2017.02101. eCollection 2017.
10
Synergistic Effect of Fluconazole and Calcium Channel Blockers against Resistant Candida albicans.氟康唑与钙通道阻滞剂对耐药白色念珠菌的协同作用。
PLoS One. 2016 Mar 17;11(3):e0150859. doi: 10.1371/journal.pone.0150859. eCollection 2016.
本文引用的文献
1
Clinical breakpoints for voriconazole and Candida spp. revisited: review of microbiologic, molecular, pharmacodynamic, and clinical data as they pertain to the development of species-specific interpretive criteria.再次探讨伏立康唑和念珠菌属的临床折点：审查微生物学、分子生物学、药效学和临床数据，以制定与特定种属相关的解释标准。
Diagn Microbiol Infect Dis. 2011 Jul;70(3):330-43. doi: 10.1016/j.diagmicrobio.2011.03.002. Epub 2011 May 4.
2
Validation of 24-hour posaconazole and voriconazole MIC readings versus the CLSI 48-hour broth microdilution reference method: application of epidemiological cutoff values to results from a global Candida antifungal surveillance program.验证 24 小时泊沙康唑和伏立康唑 MIC 读数与 CLSI 48 小时肉汤微量稀释参考方法的一致性：全球念珠菌抗真菌监测计划中应用流行病学折点值对结果的影响。
J Clin Microbiol. 2011 Apr;49(4):1274-9. doi: 10.1128/JCM.02437-10. Epub 2011 Feb 2.
3
Wild-type MIC distributions, epidemiological cutoff values and species-specific clinical breakpoints for fluconazole and Candida: time for harmonization of CLSI and EUCAST broth microdilution methods.氟康唑和念珠菌的野生型 MIC 分布、流行病学折点值和种特异性临床断点：CLSI 和 EUCAST 肉汤微量稀释方法协调的时机。
Drug Resist Updat. 2010 Dec;13(6):180-95. doi: 10.1016/j.drup.2010.09.002. Epub 2010 Nov 2.
4
Inappropriate empiric antifungal therapy for candidemia in the ICU and hospital resource utilization: a retrospective cohort study.ICU 和医院资源利用中不适当的经验性抗真菌治疗与念珠菌血症：一项回顾性队列研究。
BMC Infect Dis. 2010 Jun 3;10:150. doi: 10.1186/1471-2334-10-150.
5
Hospital resource utilization and costs of inappropriate treatment of candidemia.不适当治疗念珠菌血症的医院资源利用和成本。
Pharmacotherapy. 2010 Apr;30(4):361-8. doi: 10.1592/phco.30.4.361.
6
Clinical practice guidelines for the management of cryptococcal disease: 2010 update by the infectious diseases society of america.《隐球菌病治疗指南：美国传染病学会 2010 年更新版》
Clin Infect Dis. 2010 Feb 1;50(3):291-322. doi: 10.1086/649858.
7
Excess mortality, length of stay and cost attributable to candidaemia.念珠菌血症导致的超额死亡率、住院时间及费用
J Infect. 2009 Nov;59(5):360-5. doi: 10.1016/j.jinf.2009.08.020. Epub 2009 Sep 8.
8
Comparison of 24-hour and 48-hour voriconazole MICs as determined by the Clinical and Laboratory Standards Institute broth microdilution method (M27-A3 document) in three laboratories: results obtained with 2,162 clinical isolates of Candida spp. and other yeasts.采用临床和实验室标准协会肉汤微量稀释法（M27 - A3文件）在三个实验室中对伏立康唑24小时和48小时最低抑菌浓度（MIC）进行比较：对2162株念珠菌属及其他酵母菌临床分离株的检测结果。
J Clin Microbiol. 2009 Sep;47(9):2766-71. doi: 10.1128/JCM.00654-09. Epub 2009 Jul 1.
9
Clinical practice guidelines for the management of candidiasis: 2009 update by the Infectious Diseases Society of America.念珠菌病管理临床实践指南：美国传染病学会2009年更新版
Clin Infect Dis. 2009 Mar 1;48(5):503-35. doi: 10.1086/596757.
10
Rationale for reading fluconazole MICs at 24 hours rather than 48 hours when testing Candida spp. by the CLSI M27-A2 standard method.当按照CLSI M27-A2标准方法检测念珠菌属时，在24小时而非48小时读取氟康唑最低抑菌浓度（MIC）的理论依据。
Antimicrob Agents Chemother. 2008 Nov;52(11):4175-7. doi: 10.1128/AAC.00420-08. Epub 2008 Sep 22.
Zotero 插件