Sox2 在 C3H10T1/2 细胞中的过表达通过 Wnt 和 MAPK 信号通路抑制成骨细胞分化。
Over-expression of Sox2 in C3H10T1/2 cells inhibits osteoblast differentiation through Wnt and MAPK signalling pathways.
机构信息
Institute of Spine, Shanghai University of Traditional Chinese Medicine, 725 South Wan-Ping Road, Shanghai, 200032, People's Republic of China.
出版信息
Int Orthop. 2012 May;36(5):1087-94. doi: 10.1007/s00264-011-1368-6. Epub 2011 Oct 20.
PURPOSE
Many Sox proteins play important roles both in mesoderm and ectoderm development. It is reported that Sox2, a member of this family, is essential for the maintenance of the self-renewal of embryonic stem cells (ES) and neural stem cells (NSCs). To investigate whether Sox2 participates in mesoderm development besides ectoderm, Sox2 was introduced into C3H10T1/2 cells.
METHODS
We produced recombinant retrovirus expressing Sox2 in GP2-293t cells and infected the virus into C3H10T1/2 cells. Growth property, alkaline phosphatase (ALP) staining, mineralized nodules, osteogenic gene expression and related signal pathways were analysed and compared between Sox2-expressing cells and control cells.
RESULTS
Sox2 over-expression led to increased proliferation of C3H10T1/2 cells, activation of Wnt/β-catenin and p38MAPK pathways. When cultured in osteogenic differentiation medium, ALP and mineralized nodules formation were inhibited in Sox2 over-expressing cells with down-regulation of osteogenic gene expression as well as inhibition of Wnt/β-catenin and p38MAPK pathways.
CONCLUSIONS
All these data suggested that over-expression of Sox2 promoted proliferation and inhibited osteoblast differentiation of C3H10T1/2 cells.
目的
许多 Sox 蛋白在中胚层和外胚层发育中都发挥着重要作用。据报道，该家族的成员 Sox2 对于维持胚胎干细胞（ES）和神经干细胞（NSC）的自我更新是必不可少的。为了研究 Sox2 是否除了外胚层之外还参与中胚层发育，我们将 Sox2 导入 C3H10T1/2 细胞。
方法
我们在 GP2-293t 细胞中产生表达 Sox2 的重组逆转录病毒，并将病毒感染到 C3H10T1/2 细胞中。分析和比较 Sox2 表达细胞与对照细胞之间的生长特性、碱性磷酸酶（ALP）染色、矿化结节、成骨基因表达和相关信号通路。
结果
Sox2 的过表达导致 C3H10T1/2 细胞增殖增加，Wnt/β-catenin 和 p38MAPK 途径被激活。当在成骨分化培养基中培养时，Sox2 过表达细胞中的 ALP 和矿化结节形成受到抑制，成骨基因表达下调，Wnt/β-catenin 和 p38MAPK 途径受到抑制。
结论
所有这些数据表明，Sox2 的过表达促进了 C3H10T1/2 细胞的增殖并抑制了成骨细胞分化。
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