动力学毛细管电泳与质谱检测（KCE-MS）有助于实现无标记的基于溶液的蛋白质-小分子结合的动力学分析。
Kinetic capillary electrophoresis with mass-spectrometry detection (KCE-MS) facilitates label-free solution-based kinetic analysis of protein-small molecule binding.
机构信息
Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, Toronto, Ontario M3J 1P3, Canada.
出版信息
Chembiochem. 2011 Nov 25;12(17):2551-4. doi: 10.1002/cbic.201100617. Epub 2011 Oct 19.
PMID:22012742
Abstract
Tandem tracker: Here we introduce a method for studying the kinetics of protein-small-molecule interactions based on kinetic capillary electrophoresis (KCE) separation and MS detection. Due to the variety of KCE methods and MS modes available, the KCE-MS tandem is a highly versatile platform for label-free, solution-based kinetic studies of affinity interactions.
摘要
串联追踪器
我们在这里介绍了一种基于动力学毛细管电泳 (KCE) 分离和 MS 检测研究蛋白质-小分子相互作用动力学的方法。由于 KCE 方法和 MS 模式种类繁多，KCE-MS 串联是一种非常通用的平台，可用于无标记、基于溶液的亲和相互作用动力学研究。
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