Anal Chem. 2011 Nov 15;83(22):8387-90. doi: 10.1021/ac2026699. Epub 2011 Oct 18.
Here we demonstrate a label-free solution-based approach for studying the kinetics of biopolymer-small molecule interactions. The approach utilizes kinetic capillary electrophoresis (KCE) separation and UV light absorption detection of the unlabeled small molecule. In this proof-of-concept work, we applied KCE-UV to study kinetics of interaction between a small molecule and a DNA aptamer. From the kinetic analysis of a series of aptamers, we found that dissociation rather than binding controls the stability of the complex. Because of its label-free features and generic nature, KCE-UV promises to become a practical tool for challenging kinetic studies of biopolymer-small molecule interactions.
在这里，我们展示了一种用于研究生物聚合物-小分子相互作用动力学的无标记溶液法。该方法利用动力学毛细管电泳（KCE）分离和未标记小分子的紫外光吸收检测。在这项概念验证工作中，我们将 KCE-UV 应用于研究小分子和 DNA 适体之间相互作用的动力学。通过一系列适体的动力学分析，我们发现解离而不是结合控制复合物的稳定性。由于其无标记的特点和通用性，KCE-UV 有望成为生物聚合物-小分子相互作用的挑战性动力学研究的实用工具。
