Biosynthesis of isoprene units: Mössbauer spectroscopy of substrate and inhibitor binding to the [4Fe-4S] cluster of the LytB/IspH enzyme.
作者信息
Ahrens-Botzong Annegret, Janthawornpong Karnjapan, Wolny Juliusz A, Tambou Erasmienne Ngouamegne, Rohmer Michel, Krasutsky Sergiy, Poulter C Dale, Schünemann Volker, Seemann Myriam
机构信息
Fachbereich Physik, University of Kaiserslautern, Erwin-Schrödinger-Strasse 46, 67653 Kaiserslautern, Germany.
出版信息
Angew Chem Int Ed Engl. 2011 Dec 9;50(50):11976-9. doi: 10.1002/anie.201104562. Epub 2011 Oct 19.
Abstract
摘要
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Pyridine inhibitor binding to the 4Fe-4S protein A. aeolicus IspH (LytB): a HYSCORE Investigation.吡啶抑制剂与 4Fe-4S 蛋白 A. aeolicus IspH（LytB）的结合：HYSCORE 研究。
J Am Chem Soc. 2011 May 4;133(17):6525-8. doi: 10.1021/ja2008455. Epub 2011 Apr 12.
2
Reductive dehydroxylation of allyl alcohols by IspH protein.异戊烯基二磷酸脱水酶蛋白催化烯丙醇的还原脱羟反应。
Angew Chem Int Ed Engl. 2010 Nov 15;49(47):8802-9. doi: 10.1002/anie.201000833.
3
Inhibition of the Fe(4)S(4)-cluster-containing protein IspH (LytB): electron paramagnetic resonance, metallacycles, and mechanisms.Fe(4)S(4)- 簇蛋白 IspH（LytB）的抑制作用：电子顺磁共振、金属环和机制。
J Am Chem Soc. 2010 May 19;132(19):6719-27. doi: 10.1021/ja909664j.
4
Bioorganometallic mechanism of action, and inhibition, of IspH.IspH 的生物有机金属作用机制及抑制作用。
Proc Natl Acad Sci U S A. 2010 Mar 9;107(10):4522-7. doi: 10.1073/pnas.0911087107. Epub 2010 Feb 19.
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Probing the reaction mechanism of IspH protein by x-ray structure analysis.通过 X 射线结构分析探究 IspH 蛋白的反应机制。
Proc Natl Acad Sci U S A. 2010 Jan 19;107(3):1077-81. doi: 10.1073/pnas.0913045107. Epub 2009 Dec 28.
6
Isoprenoid biosynthesis via the MEP pathway: in vivo Mössbauer spectroscopy identifies a [4Fe-4S]2+ center with unusual coordination sphere in the LytB protein.异戊烯基生物合成途径：体内穆斯堡尔谱学鉴定 LytB 蛋白中具有异常配位环境的 [4Fe-4S]2+中心。
J Am Chem Soc. 2009 Sep 23;131(37):13184-5. doi: 10.1021/ja9012408.
7
Structure of active IspH enzyme from Escherichia coli provides mechanistic insights into substrate reduction.来自大肠杆菌的活性IspH酶的结构为底物还原提供了机制上的见解。
Angew Chem Int Ed Engl. 2009;48(31):5756-9. doi: 10.1002/anie.200900548.
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Structure of (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase, the terminal enzyme of the non-mevalonate pathway.（E）-4-羟基-3-甲基-丁-2-烯基二磷酸还原酶的结构，非甲羟戊酸途径的末端酶。
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Mechanistic studies of IspH in the deoxyxylulose phosphate pathway: heterolytic C-O bond cleavage at C4 position.脱氧木酮糖磷酸途径中IspH的机制研究：C4位的异裂碳-氧键断裂
J Am Chem Soc. 2008 Feb 20;130(7):2164-5. doi: 10.1021/ja710245d. Epub 2008 Jan 25.
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Isoprenoid biosynthesis as a novel target for antibacterial and antiparasitic drugs.类异戊二烯生物合成作为抗菌和抗寄生虫药物的新靶点。
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