Department of Geriatric Surgery, Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Cancer Biomark. 2010;8(3):113-21. doi: 10.3233/DMA-2011-0812.
In this study, we investigated the expressions of Msi-1 and ALDH1 in gallbladder adenocarcinoma (n=100), peritumoral tissues (n=46), adenomatous polyp (n=15), and chronic cholecystitis (n=35) using immunohistochemical method. The percentage of cases with positive Msi-1 and ALDH1 expression were significantly higher in gallbladder adenocarcinoma than that in peritumoral tissues, adenomatous polyp and chronic cholecystitis (ps < 0.01). The expression of Msi-1 and ALDH1 was significantly associated with differentiation, tumor mass, lymph node metastasis and invasion of adenocarcinoma. The expression of Msi-1 and ALDH1 was found to be highly consistent in gallbladder adenocarcinoma (p < 0.01). Univariate Kaplan-Meier analysis showed a negative correlation between Msi-1 (p=0.042) or ALDH1 (p< 0.001) expression with overall survival. The average survival time of patients with both low or no Msi-1 expression and ALDH1 expression was significantly longer than patients with the other three subtypes (p< 0.001). Multivariate Cox regression analysis showed that positive expression of Msi-1 or ALDH1 (p=0.016, p=0.006, respectively) was an independent bad-prognostic predictor in gallbladder adenocarcinoma. Our study suggested that Msi-1 and/or ALDH1 expression might be closely related to the carcinogenesis, progression, clinical biological behaviors, and prognosis of gallbladder adenocarcinoma.
本研究采用免疫组织化学方法检测了 100 例胆囊腺癌、46 例癌旁组织、15 例腺瘤性息肉和 35 例慢性胆囊炎中 Msi-1 和 ALDH1 的表达。胆囊腺癌中 Msi-1 和 ALDH1 阳性表达的病例百分比明显高于癌旁组织、腺瘤性息肉和慢性胆囊炎（p<0.01）。Msi-1 和 ALDH1 的表达与胆囊腺癌的分化、肿瘤大小、淋巴结转移和浸润显著相关。胆囊腺癌中 Msi-1 和 ALDH1 的表达高度一致（p<0.01）。单因素 Kaplan-Meier 分析显示，Msi-1（p=0.042）或 ALDH1（p<0.001）表达与总生存呈负相关。同时低表达或不表达 Msi-1 和 ALDH1 的患者平均生存时间明显长于其他三种亚型（p<0.001）。多因素 Cox 回归分析显示，Msi-1 或 ALDH1 的阳性表达（p=0.016，p=0.006）是胆囊腺癌的独立预后不良预测因子。本研究提示 Msi-1 和/或 ALDH1 的表达可能与胆囊腺癌的发生、发展、临床生物学行为及预后密切相关。
