Xiao Ruihai, Yu Yi, Shen Shaochen, Liu Fei, Kuang Renrui
Department of Urology, The Second Affiliated Hospital of Nanchang University Nanchang, Jiangxi Province, People's Republic of China.
Int J Clin Exp Pathol. 2019 Jan 1;12(1):313-319. eCollection 2019.
Musashi1 (MSI1) has been reported to be involved in cancer development and progression. The biologic role of MSI1 in renal cell carcinoma (RCC), however, remains unknown.
Expression of MSI1 in normal kidney cells and kidney cancer cells were measured by real-time PCR. In addition, MSI1 expression in 20 paired kidney cancer and non-cancerous tissues were quantified using real-time PCR. Furthermore, the expression of MSI1 in 115 kidney cancer samples was detected to analyze the correlations between MSI1 expression and the clinicopathological features of RCC patients. The biological function of MSI1 on tumor cell invasion and migration were explored through wound healing and transwell migration assays.
MSI1 was significantly upregulated in renal cancer cells and tissues compared with normal kidney cells and tissues. High levels of MSI1 were positively associated with tumor stage (P=0.002) and distant metastasis (P=0.013) of RCC patients. Patients with higher MSI1 expression had a significantly poorer overall and recurrence-free survival time (P=0.019 and P=0.012, respectively) than patients with low MSI1 expression. Multivariate analysis showed that MSI1 overexpression was an independent prognostic indicator (P=0.009 and P=0.015, respectively) for the survival of RCC patients. Ablation of MSI1 inhibited the invasion and metastasis of renal cancer cells.
Our results suggest that MSI1 expression is upregulated in RCC, and that MSI1 plays an important role in promoting cell invasion and metastasis of RCC.
据报道，Musashi1（MSI1）参与癌症的发生和发展。然而，MSI1在肾细胞癌（RCC）中的生物学作用尚不清楚。
通过实时PCR检测正常肾细胞和肾癌细胞中MSI1的表达。此外，使用实时PCR对20对肾癌组织和癌旁组织中的MSI1表达进行定量。进一步检测115例肾癌样本中MSI1的表达，以分析MSI1表达与RCC患者临床病理特征之间的相关性。通过伤口愈合实验和Transwell迁移实验探讨MSI1对肿瘤细胞侵袭和迁移的生物学功能。
与正常肾细胞和组织相比，MSI1在肾癌细胞和组织中显著上调。MSI1高表达与RCC患者的肿瘤分期（P=0.002）和远处转移（P=0.013）呈正相关。MSI1表达较高的患者的总生存期和无复发生存期明显低于MSI1表达较低的患者（分别为P=0.019和P=0.012）。多变量分析表明，MSI1过表达是RCC患者生存的独立预后指标（分别为P=0.009和P=0.015）。敲除MSI1可抑制肾癌细胞的侵袭和转移。
我们的结果表明，RCC中MSI1表达上调，且MSI1在促进RCC细胞侵袭和转移中起重要作用。
