Department of Oral and Maxillofacial Pathology, Mar Baselious Dental College and Hospital, Kothamangalam, Kerala, India.
Cancer Biomark. 2010;8(3):155-60. doi: 10.3233/CBM-2011-0207.
Prolonged production of the free radical, nitric oxide (NO) by the enzyme inducible nitric oxide synthase (iNOS) plays an important role in tumour progression by promoting angiogenesis, invasion and inducing mutation in tumour suppressor gene. The purpose of this study is to evaluate the expression and intensity of iNOS in normal oral mucosa, precancer and oral squamous cell carcinoma (OSCC).
An immunohistochemical study was performed using rabbit monoclonal antibody to iNOS on archival formalin fixed, paraffin embedded tissues of 5 normal oral mucosal samples, 10 Leukoplakia, 10 oral submucous fibrosis (OSMF) and 15 OSCC of different grades.
A statistical significant difference was found between normal oral mucosa, precancer and OSCC in expression of iNOS (p value 0.0015). However, there was no statistically significant difference between the expressions of iNOS within premalignant groups (p value 0.5647) and histological sub-groups of OSCC (p value-0.5647). There was no statistically significant difference in the intensity of iNOS expression within the precancer group, OSCC sub-groups and between precancer and OSCC (p value-0.623).
The orderly increase in the expression of iNOS from normal, through precancer, to OSCC suggests the essential role played by iNOS in epithelial transformation and tumour formation.
诱导型一氧化氮合酶（iNOS）产生的自由基一氧化氮（NO）的持续产生在肿瘤进展中起着重要作用，它通过促进血管生成、侵袭和诱导肿瘤抑制基因突变来实现。本研究旨在评估 iNOS 在正常口腔黏膜、癌前病变和口腔鳞状细胞癌（OSCC）中的表达和强度。
使用兔单克隆抗体 iNOS 对 5 例正常口腔黏膜样本、10 例白斑、10 例口腔黏膜下纤维性变（OSMF）和 15 例不同分级的 OSCC 的福尔马林固定、石蜡包埋组织进行免疫组织化学研究。
iNOS 的表达在正常口腔黏膜、癌前病变和 OSCC 之间存在统计学显著差异（p 值 0.0015）。然而，在癌前病变组内（p 值 0.5647）和 OSCC 的组织学亚组之间（p 值 0.5647），iNOS 的表达无统计学显著差异。癌前病变组、OSCC 亚组和癌前病变与 OSCC 之间 iNOS 表达强度无统计学显著差异（p 值 0.623）。
iNOS 的表达从正常、癌前病变到 OSCC 的有序增加表明 iNOS 在上皮转化和肿瘤形成中发挥了重要作用。
