Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Biol Trace Elem Res. 2011 Dec;144(1-3):426-35. doi: 10.1007/s12011-011-9119-5. Epub 2011 Jun 17.
The effect of potassium dichromate (K(2)Cr(2)O(7)), a hexavalent chromium compound, on human erythrocytes was studied under in vitro conditions. Incubation of erythrocytes with different concentrations of K(2)Cr(2)O(7) resulted in marked hemolysis in a concentration-dependent manner. K(2)Cr(2)O(7) treatment also caused significant increase in protein oxidation, lipid peroxidation and decrease in total sulfhydryl content, indicating that it causes oxidative stress in human erythrocytes. However, there was no concomitant nitrosative stress as the nitric oxide levels in hemolysates from K(2)Cr(2)O(7)-treated erythrocytes were lower than in control. Exposure of erythrocytes to K(2)Cr(2)O(7) decreased the activities of catalase, glutathione peroxidase, thioredoxin reductase, glucose-6-phosphate dehydrogenase, and glutathione reductase, whereas the activities of Cu-Zn superoxide dismutase and glutathione S-transferase were increased. These results show that K(2)Cr(2)O(7) induces oxidative stress and alters the antioxidant defense mechanism of human erythrocytes.
六价铬化合物重铬酸钾（K2Cr2O7）对人红细胞的体外影响研究。红细胞与不同浓度的 K2Cr2O7 孵育，以浓度依赖的方式导致明显的溶血。K2Cr2O7 处理还导致蛋白质氧化、脂质过氧化显著增加，总巯基含量降低，表明它导致人红细胞的氧化应激。然而，没有伴随的硝化应激，因为 K2Cr2O7 处理的红细胞溶血物中的一氧化氮水平低于对照。红细胞暴露于 K2Cr2O7 会降低过氧化氢酶、谷胱甘肽过氧化物酶、硫氧还蛋白还原酶、葡萄糖-6-磷酸脱氢酶和谷胱甘肽还原酶的活性，而铜锌超氧化物歧化酶和谷胱甘肽 S-转移酶的活性则增加。这些结果表明，K2Cr2O7 诱导氧化应激并改变人红细胞的抗氧化防御机制。
