School of Forestry and Resource Conservation, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Daan, Taipei 10617 Taiwan.
Am J Bot. 2011 Nov;98(11):e326-9. doi: 10.3732/ajb.1100220. Epub 2011 Oct 19.
Microsatellite loci were developed in Sassafras randaiense, an endemic tree species in Taiwan, to investigate the conservation genetics of this invaluable plant that is at risk for continuing population decline.
Using a magnetic bead enrichment method, 27 primer pairs amplifying di-, tri, and tetranucleotide repeats were developed. Across the populations tested, 16 primer sets showed polymorphism, with an average of 4.06 alleles per polymorphic locus. Twenty of the 27 developed primer sets were also successfully amplified in its congener S. tzumu from China.
The new primer sets described here are a powerful tool for investigating the spatial patterns of genetic diversity and for designing effective management strategies for this invaluable plant.
本研究开发了台湾特有树种红皮糙果茶的微卫星标记，以探讨这种极具价值的植物的保护遗传学，该植物正面临持续种群减少的风险。
使用磁珠富集法，开发了 27 对扩增二、三、四核苷酸重复的引物对。在所测试的种群中，16 对引物表现出多态性，每个多态性位点平均有 4.06 个等位基因。在其中国的同属种红皮糙果茶中，27 对开发引物中有 20 对也成功扩增。
本文所述的新引物对是研究遗传多样性空间格局和为这种极具价值的植物设计有效管理策略的有力工具。
