Goudsmit J, Geelen J, Keulen W, Notermans D, Kuiken C, Ramautarsing C, Smit L, Koole L, van Genderen M, Buck H
Human Retrovirus Laboratory, Academic Medical Center, University of Amsterdam, The Netherlands.
AIDS. 1990 Jun;4(6):559-64. doi: 10.1097/00002030-199006000-00010.
The HIV-1 isolate CBL-4 (RUT), originating from Tanzania, was characterized using a comprehensive virus-typing system. This system included sequence analysis of the region coding for the neutralization domain in the third variable region (V3) of the external envelope and of the tat responsive (TAR) region after polymerase chain reaction (PCR) amplification of these sequences from cellular DNA in the CBL-4 (RUT) producer line. Based on independent cluster analysis of TAR and V3 sequences the CBL-4 (RUT) virus was positioned closest to the Z6 (and ELI) African virus family. The V3 amino acid sequence on the surface of the virus particle was confirmed by the inhibition of neutralization of CBL-4 (RUT) by a synthetic peptide derived from the nucleic acid sequence. Using antisense phosphate-methylated DNA covering the TAR loop region of LAV-1/HTLV-IIIB, inhibition of HTLV-IIIB and HTLV-IIIRF infection was seen, whereas no inhibition was observed for CBL-4 (RUT), indicating two or more mismatches in the TAR loop region, a characteristic shared with Z6 virus, but not with ELI. We propose a virus-typing system based on sequence analysis confirmed by virus neutralization with a peptide binding antibody and inhibition by antisense phosphate-methylated DNA to group viruses for laboratory use and vaccine design.
源自坦桑尼亚的HIV-1分离株CBL-4（RUT），使用综合病毒分型系统进行了特征分析。该系统包括在对CBL-4（RUT）产生细胞系中的细胞DNA进行这些序列的聚合酶链反应（PCR）扩增后，对外包膜第三可变区（V3）中编码中和结构域的区域以及tat反应区（TAR）进行序列分析。基于对TAR和V3序列的独立聚类分析，CBL-4（RUT）病毒最接近Z6（和ELI）非洲病毒家族。通过源自核酸序列的合成肽对CBL-4（RUT）中和作用的抑制，证实了病毒颗粒表面的V3氨基酸序列。使用覆盖LAV-1/HTLV-IIIB的TAR环区域的反义磷酸甲基化DNA，观察到对HTLV-IIIB和HTLV-IIIRF感染有抑制作用，而对CBL-4（RUT）未观察到抑制作用，这表明TAR环区域存在两个或更多错配，这是与Z6病毒共有的特征，但与ELI病毒不同。我们提出了一种基于序列分析的病毒分型系统，该分析通过肽结合抗体的病毒中和以及反义磷酸甲基化DNA的抑制作用来证实，用于对病毒进行分组以供实验室使用和疫苗设计。
