Gan Ning, Jia Liyong, Zheng Lei
State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
J Autom Methods Manag Chem. 2011;2011:957805. doi: 10.1155/2011/957805. Epub 2011 Oct 13.
One novel electrochemical immunosensor was constructed by immobilizing capture antibody of alpha-fetoprotein (AFP Ab(1)) on a nafion/nanogold-particle modified glassy carbon electrode. With a sandwich immunoassay, one DNA-derived magnetic nanoprobe, simplified as DNA/(ZMPs-HRP-AFP Ab(2))(n), was employed for the detection of AFP. The fabricated procedure of the proposed biosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The performance and factors influencing the performance of the biosensor were also evaluated. Under optimal conditions, the developed biosensor exhibited a well-defined electrochemical behavior toward the reduction of AFP ranging from 0.01 to 200 ng/mL with a detection limit of 4 pg/mL (S/N = 3). The biosensor was applied to the determination of AFP in serum with satisfactory results. It is important to note that the sandwich nanochainmodified electro-immunosensor provided an alternative substrate for the immobilization of other tumor markers.
通过将甲胎蛋白捕获抗体（AFP Ab(1)）固定在全氟磺酸/纳米金颗粒修饰的玻碳电极上构建了一种新型电化学免疫传感器。采用夹心免疫分析法，一种简化为DNA/(ZMPs-HRP-AFP Ab(2))(n)的DNA衍生磁性纳米探针用于检测AFP。通过循环伏安法和电化学阻抗谱对所提出的生物传感器的制备过程进行了表征。还评估了生物传感器的性能及影响其性能的因素。在最佳条件下，所开发的生物传感器对0.01至200 ng/mL的AFP还原表现出明确的电化学行为，检测限为4 pg/mL（S/N = 3）。该生物传感器应用于血清中AFP的测定，结果令人满意。需要注意的是，夹心纳米链修饰的电免疫传感器为固定其他肿瘤标志物提供了一种替代基质。
