一种基于信号放大技术的电化学夹心免疫传感器用于甲胎蛋白的测定

An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein.

作者信息

Shen Changming, Wang Lin, Zhang Hongyan, Liu Shaojuan, Jiang Jianwei

机构信息

Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, China.

Institute of Cancer and Basic Medicine (IBMC), Chinese Academy of Sciences, Hangzhou, China.

出版信息

Front Chem. 2020 Sep 16;8:589560. doi: 10.3389/fchem.2020.589560. eCollection 2020.

The synthesis of Au nanocubes is used to label alpha-fetoprotein antibody (anti-AFP) and horseradish peroxidase (HRP) to form an immune complex for antibody detection. Graphene oxide-methylene blue-gold nanoparticles (GO-MB-AuNPs) nanocomposites were used as the immunosensing platform. This proposed sandwich-type immunoassay shows good performance. This method establishes a feasible amperometric immunoassay method for sensitive analysis of AFP in serum samples. Under the optimal experimental conditions, the DPV current response of the immunosensor is proportional to the logarithmic value of the AFP concentration. The linear detection range can achieve to 0.005-20 ng/mL with a detection limit of 1.5 pg/mL. The proposed immunosensor has good precision, selectivity and stability, and can be used for AFP determination in clinical tests.

金纳米立方体的合成用于标记甲胎蛋白抗体（抗 AFP）和辣根过氧化物酶（HRP），以形成用于抗体检测的免疫复合物。氧化石墨烯-亚甲基蓝-金纳米颗粒（GO-MB-AuNPs）纳米复合材料用作免疫传感平台。这种提出的夹心型免疫分析表现出良好的性能。该方法建立了一种可行的安培免疫分析方法，用于血清样本中 AFP 的灵敏分析。在最佳实验条件下，免疫传感器的差分脉冲伏安法（DPV）电流响应与 AFP 浓度的对数值成正比。线性检测范围可达 0.005 - 20 ng/mL，检测限为 1.5 pg/mL。所提出的免疫传感器具有良好的精密度、选择性和稳定性，可用于临床检测中 AFP 的测定。