Chen Yiyan, Chen Xiaoping, Lin Jianwei, Zhuang Yafeng, Han Zhizhong, Chen Jinghua
School of Pharmacy, Fujian Medical University, Fuzhou 350122, China.
Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, Fuzhou 350122, China.
Micromachines (Basel). 2022 Apr 26;13(5):673. doi: 10.3390/mi13050673.
Black phosphorus nanosheets (BPNSs) were synthesized with liquid exfoliation combined with the ultrasonic method and loaded with Fe by simply mixing. The morphology, structure and electrochemical properties of the synthesized Fe/BPNSs were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV), etc. The load of Fe can improve the electrochemical performance of BPNSs and enhance the sensitivity of the detection. Additionally, Fe/BPNSs display good biocompatibility. In this study, immunosensors based on Fe/BPNSs were constructed to detect alpha-fetoprotein (AFP). The detection is due to the specific binding between the AFP antigen and antibody on the surface of the immunosensors, which can reduce the current response of Fe/BPNSs. The immunosensors have a good linear relationship in the range of 0.005 ng·mL to 50 ng·mL, and the detection limit is 1.2 pg·mL. The results show that surface modification with metal ions is a simple and effective way to improve the electrochemical properties of BPNSs, which will broaden the prospects for the future application of BPNSs in the electrochemical field.
采用液相剥离结合超声法合成了黑磷纳米片（BPNSs），并通过简单混合负载了铁。通过透射电子显微镜（TEM）、原子力显微镜（AFM）、拉曼光谱、X射线光电子能谱（XPS）和循环伏安法（CV）等对合成的Fe/BPNSs的形貌、结构和电化学性能进行了表征。铁的负载可以改善BPNSs的电化学性能并提高检测灵敏度。此外，Fe/BPNSs表现出良好的生物相容性。在本研究中，构建了基于Fe/BPNSs的免疫传感器用于检测甲胎蛋白（AFP）。检测是由于免疫传感器表面的AFP抗原与抗体之间的特异性结合，这会降低Fe/BPNSs的电流响应。该免疫传感器在0.005 ng·mL至50 ng·mL范围内具有良好的线性关系，检测限为1.2 pg·mL。结果表明，用金属离子进行表面修饰是改善BPNSs电化学性能的一种简单有效的方法，这将拓宽BPNSs在电化学领域未来应用的前景。
