健康牙龈与中度至重度慢性牙周炎中IFNγ、IL4和IL17细胞因子的比较评估
Comparative Evaluation of IFNγ, IL4 and IL17 Cytokines in Healthy Gingiva and Moderate to Advanced Chronic Periodontitis.
作者信息
Behfarnia Parichehr, Birang Reza, Andalib Ali Reza, Asadi Shahram
机构信息
Associate Professor, Department of Periodontics, School of Dentistry and Torabinejad Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Dent Res J (Isfahan). 2010 Summer;7(2):45-50.
BACKGROUND
Considering the role of T cells in the pathogenesis of periodontitis, the purpose of this study was to compare the amount of IFNγ, IL4 and IL17 in advanced periodontal lesions with healthy gum areas to determine each TH1, TH2 and TH17 cells activity in comparison with each other and finally, to compare the value and the role of humoral, cell mediate and autoimmune responses.
METHODS
In this descriptive analytical study, those with moderate to advanced periodontitis, having pocket with 4 to 7 mm in depth, were randomly selected. After preparing the healthy and affected sample tissues, the amount of the specific antibody in I IFNγ, IL4 and IL17 cytokines were measured using ELISA method and were compared between the two groups. The findings were analyzed using the software and descriptive statistical methods and Pearson correlation statistical analysis (α = 0.05).
RESULTS
This study was performed on 37 patients with moderate to severe periodontitis and 22 healthy individuals without any periodontal disease. IL4 and IFNγ levels in the patients with chronic periodontitis compared to those of healthy gingival samples showed a significant reduction (P > 0.05) whereas the amount of IL17 in tissue samples of chronic periodontitis compared to healthy gums had a significant increase (P < 0.05).
CONCLUSION
It appears that in the periodontitis pathogenesis, as well as TH1 and TH2 responses, IL17 causes the host immunological response to the periodontal pathogenesis.
背景
考虑到T细胞在牙周炎发病机制中的作用，本研究的目的是比较晚期牙周病变与健康牙龈组织中γ干扰素（IFNγ）、白细胞介素4（IL4）和白细胞介素17（IL17）的含量，以确定辅助性T细胞1（TH1）、辅助性T细胞2（TH2）和辅助性T细胞17（TH17）的活性，并相互比较，最终比较体液免疫、细胞介导免疫和自身免疫反应的价值及作用。
方法
在这项描述性分析研究中，随机选取中度至重度牙周炎患者，其牙周袋深度为4至7毫米。制备健康和患病变组织样本后，采用酶联免疫吸附测定（ELISA）法测量IFNγ、IL4和IL17细胞因子中的特异性抗体含量，并在两组之间进行比较。使用软件和描述性统计方法以及Pearson相关统计分析（α = 0.05）对研究结果进行分析。
结果
本研究对37例中度至重度牙周炎患者和22例无任何牙周疾病的健康个体进行。与健康牙龈样本相比，慢性牙周炎患者的IL4和IFNγ水平显著降低（P > 0.05），而慢性牙周炎组织样本中的IL17含量与健康牙龈相比显著增加（P < 0.05）。
结论
在牙周炎发病机制中，似乎除了TH1和TH2反应外，IL17也会引发宿主对牙周发病机制的免疫反应。
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