Institute for Research in Molecular Medicine (INFORMM), University Sains Malaysia, Minden, Penang, Malaysia.
Eur Rev Med Pharmacol Sci. 2011 Sep;15(9):1020-6.
The Gracilaria (G.) sp are widely used in the traditional medicine in Malaysia. The methanol extract of Gracilaria changii B.M. Xia & I.A. Abbott (Gracilariaciae) was evaluated for antiyeast activity against Candida albicans (Berkhout).
The antiyeast activities were studied by using disc diffusion method and broth dilution method. The effect of the extract on the growth profile of the yeast was also examined via time-kill assay. In addition, the in situ antiyeast activity was studied by microscopic observations using scanning electron microscopy (SEM), transmission electron microscopy (TEM) to determine the major alterations in the microstructure of Candida (C.) albicans.
The extract showed a favourable antimicrobial activity against C albicans with a minimum inhibitory concentration (MIC) value of 1.56 mg/mL. The main abnormalities noted from the SEM and TEM studies were the internal shrinkage of cell, disorganization within the cell cytoplasm and complete collapse of the yeast cells after 36 h of exposure to the extract. The time-kill assay suggested that the G. changii extract significantly inhibited C. albicans growth and it also exhibited prolonged antiyeast activity against the C albicans.
The extract has shown in vitro fungicidal properties against C. albicans and should be investigated for its therapeutic potential.
马来西亚传统医学广泛使用江蓠（G.）属植物。本研究评估了江蓠 Changii B.M. Xia & I.A. Abbott（江蓠科）甲醇提取物对白色念珠菌（Berkhout）的抗真菌活性。
采用圆盘扩散法和肉汤稀释法研究抗真菌活性。通过时间杀伤试验还研究了提取物对酵母生长曲线的影响。此外，通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）的微观观察研究了原位抗真菌活性，以确定白色念珠菌（C.）的微观结构的主要变化。
提取物对白色念珠菌具有良好的抗菌活性，最小抑菌浓度（MIC）值为 1.56 mg/mL。SEM 和 TEM 研究中观察到的主要异常是细胞内部收缩、细胞质内组织紊乱以及暴露于提取物 36 小时后酵母细胞完全崩溃。时间杀伤试验表明，江蓠提取物显著抑制白色念珠菌的生长，并且对白色念珠菌表现出延长的抗真菌活性。
该提取物显示出对白色念珠菌的体外杀菌特性，应进一步研究其治疗潜力。
