Sahgal G, Ramanathan S, Sasidharan S, Mordi M N, Ismail S, Mansor S M
Centre for Drug Research, University Sains Malaysia, 11800 Penang, Malaysia.
Trop Biomed. 2011 Apr;28(1):132-7.
Swietenia mahogani crude methanolic (SMCM) seed extract was investigated for the antifungal activity against Candida albicans which has not been evaluated previously. The antifungal activity was evaluated against C. albicans via disk diffusion, minimum inhibition concentration (MIC), scanning electron microscope (SEM), transmission electron microscope (TEM) and time killing profile. The MIC value of SMCM seed extract is 12.5 mg/ml. The SEM and TEM findings showed there is morphological changes and cytological destruction of C. albicans at the MIC value. Animal model was used to evaluate the in vivo antifungal activity of SMCM seed extract. The colony forming unit (CFU) were calculated per gram of kidney sample and per ml of blood sample respectively for control, curative and ketaconazole treated groups. There was significant reduction for the CFU/ml of blood and CFU/g of kidney. This indicated that the extract was observed to be effective against C. albicans in vitro and in vivo conditions.
对桃花心木粗甲醇（SMCM）种子提取物针对白色念珠菌的抗真菌活性进行了研究，此前尚未对其进行过评估。通过纸片扩散法、最低抑菌浓度（MIC）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和时间杀菌曲线评估了对白色念珠菌的抗真菌活性。SMCM种子提取物的MIC值为12.5毫克/毫升。扫描电子显微镜和透射电子显微镜的结果表明，在MIC值时白色念珠菌存在形态变化和细胞学破坏。使用动物模型评估SMCM种子提取物的体内抗真菌活性。分别计算对照组、治疗组和酮康唑治疗组每克肾脏样本和每毫升血液样本中的菌落形成单位（CFU）。血液中的CFU/毫升和肾脏中的CFU/克均有显著降低。这表明该提取物在体外和体内条件下对白色念珠菌均有效。
