Shu Qi-jin, Li Ping, Wang Bin-bin
Department of Oncology, The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Sep;31(9):1243-7.
To investigate the apoptosis inducing effects of aqueous extract of Taxus chinensis (AETC) in human lung cancer cell A549 and its molecular mechanisms.
Using MTT assay, the inhibition of AETC on the proliferation of A549 cells was detected. The apoptosis was detected by light and electron microscopy, and Annexin V-FITC/PI staining. The expressions of ERK1/2, JNK1/2, and survivin were analyzed by Western blot.
AETC could inhibit the proliferation of A549 cells. Obvious apoptotic cells could be seen under light and electron microscope in AETC treated A549 cells. AETC's induction on the cell apoptosis was further confirmed by Annexin V-FITC/PI labeled quantitative detection. Western blot assay showed that AETC could decrease the survivin expressions. AETC showed no effects on ERK1/2 or JNK1/2 protein expressions.
AETC could significantly inhibit the proliferation of A549 cells and induce the apoptosis, which was mainly achieved through regulating the expressions of survivin.
探讨中国红豆杉水提取物（AETC）对人肺癌细胞A549的凋亡诱导作用及其分子机制。
采用MTT法检测AETC对A549细胞增殖的抑制作用。通过光镜和电镜以及Annexin V-FITC/PI染色检测细胞凋亡。采用蛋白质印迹法分析ERK1/2、JNK1/2和生存素的表达。
AETC可抑制A549细胞的增殖。在光镜和电镜下可观察到经AETC处理的A549细胞中有明显的凋亡细胞。Annexin V-FITC/PI标记定量检测进一步证实了AETC对细胞凋亡的诱导作用。蛋白质印迹分析表明，AETC可降低生存素的表达。AETC对ERK1/2或JNK1/2蛋白表达无影响。
AETC可显著抑制A549细胞的增殖并诱导其凋亡，这主要是通过调节生存素的表达来实现的。
