Miniaturisation pour l'Analyse, la Synthèse & la Protéomique (MSAP), USR CNRS 3290, Université de Lille 1 Sciences et Technologies, Villeneuve d'Ascq, France.
Anal Chem. 2011 Dec 15;83(24):9431-7. doi: 10.1021/ac201978j. Epub 2011 Nov 17.
This study proposes a proteomic-based strategy for the identification of the origin species of glues used as binding media and adhesives in artworks. The methodology, based on FTICR high resolution mass spectrometry, was evaluated on glues from different animal origin (i.e., bovine, rabbit, and fish). The analysis of the peptide mixture resulting from the enzymatic hydrolysis of the proteins led to the identification of species-specific peptides. Up to 15 specific peptides were identified for the bovine species and three for the rabbit species and, in the case of sturgeon glue, three fish-specific peptides were found by sequence homology to the rainbow trout. Then, the method was applied to authenticate different rabbit skin glue samples, including a 100 year-old sample named "Colle à Doreurs" coming from the "Maison Totin-Frères". For this sample, two specific peptides of rabbit collagen were identified. To evaluate the method in a complex matrix, model paints composed of lead white, linseed oil, and animal glue were prepared. Species-specific peptides were identified in each paint sample. Finally, a gilt sample from St Maximin church dating from the eighteenth century was analyzed, and 13 peptides specific to bovine collagens were identified starting from very low sample amount (50 μg).
本研究提出了一种基于蛋白质组学的策略，用于鉴定艺术作品中用作黏合剂和胶粘剂的胶水的起源物种。该方法基于 FTICR 高分辨率质谱，在不同动物来源的胶水（即牛、兔和鱼）上进行了评估。对蛋白质酶解产生的肽混合物进行分析，得到了物种特异性肽。鉴定出牛物种的多达 15 种特异性肽，兔物种的 3 种特异性肽，而在鲟鱼胶的情况下，通过与虹鳟鱼的序列同源性发现了 3 种鱼类特异性肽。然后，该方法应用于鉴定不同的兔皮胶样品，包括来自“Maison Totin-Frères”的一种名为“Colle à Doreurs”的百年老样品。对于该样品，鉴定出了两种兔胶原蛋白的特异性肽。为了在复杂基质中评估该方法，制备了由铅白、亚麻籽油和动物胶组成的模型漆。在每个漆样品中都鉴定出了物种特异性肽。最后，对来自圣马克西米恩教堂的一个镀金样本进行了分析，该样本来自 18 世纪，从非常少量的样品（50μg）中鉴定出了 13 种牛胶原蛋白特异性肽。
