普洱熟茶和 GABA 减轻了海人酸诱导的癫痫持续状态中的氧化应激。

BACKGROUND

Pu-Erh tea is one of the most-consumed beverages due to its taste and the anti-anxiety-producing effect of the gamma-aminobutyric acid (GABA) if contains. However the protective effects of Pu-Erh tea and its constituent, GABA to kainic acid (KA)-induced seizure have not been fully investigated.

METHODS

We analyzed the effect of Pu-Erh tea leaf (PETL) and GABA on KA-induced neuronal injury in vivo and in vitro.

RESULTS

PETL and GABA reduced the maximal seizure classes, predominant behavioral seizure patterns, and lipid peroxidation in male FVB mice with status epilepticus. PETL extracts and GABA were effective in protecting KA-treated PC12 cells in a dose-dependent manner and they decreased Ca(2+) release, ROS production and lipid peroxidation from KA-stressed PC12 cells. Western blot results revealed that mitogen-activated protein kinases (MAPKs), RhoA and cyclo-oxygenase-2 (COX-2) expression were increased in PC12 cells under KA stress, and PETL and GABA significantly reduced COX-2 and p38 MAPK expression, but not that of RhoA. Furthermore, PETL and GABA reduced PGE(2) production from KA-induced PC12 cells.

CONCLUSIONS

Taken together, PETL and GABA have neuroprotective effects against excitotoxins that may have clinical applications in epilepsy.

背景

普洱茶由于其口感和所含的γ-氨基丁酸（GABA）的抗焦虑作用，是消费最多的饮料之一。然而，普洱茶及其成分 GABA 对海人酸（KA）诱导的癫痫发作的保护作用尚未得到充分研究。

方法

我们分析了普洱茶叶（PETL）和 GABA 对体内和体外 KA 诱导的神经元损伤的影响。

结果

PETL 和 GABA 减少了 FVB 雄性癫痫持续状态小鼠的最大癫痫发作等级、主要行为癫痫发作模式和脂质过氧化。PETL 提取物和 GABA 以剂量依赖的方式有效保护 KA 处理的 PC12 细胞，降低 KA 应激 PC12 细胞中的 Ca（2+）释放、ROS 产生和脂质过氧化。Western blot 结果表明，MAPKs、RhoA 和环加氧酶-2（COX-2）在 KA 应激下 PC12 细胞中的表达增加，PETL 和 GABA 显著降低 COX-2 和 p38 MAPK 的表达，但不降低 RhoA 的表达。此外，PETL 和 GABA 减少了 KA 诱导的 PC12 细胞中 PGE（2）的产生。