Farwa Umme, Abbasi Shahid Ahmad, Mirza Irfan Ali, Amjad Alina, Ikram Aamer, Malik Nasrullah, Hanif Faisal
Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi.
J Coll Physicians Surg Pak. 2011 Oct;21(10):597-600. doi: 10.2011/JCPSP.597600.
To determine the role and pattern of non-dermatophyte moulds as causative agents of onychomycosis.
Case series.
Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from November 2009 to July 2010.
Nail clippings and nail scrapings were obtained from abnormal looking nails with treatment and detection failure for onychomycosis. Microscopic (40% potassium hydroxide mounts) examination and culture on Sabouraud's dextrose agar (SDA), SDA containing chloramphenicol, and SDA containing actidione and chloramphenicol were used for species identification.
Non-dermatophyte moulds were isolated from 32 out of the total 47 culture positive cases (68%). Alternaria alternata was the commonest species (46%). Dermatophytes were isolated from only 7 patients (15%) belonging to genus Trichophyton. Yeasts were isolated in 8 (17%). There was no fungal growth in 53% of cases.
The non-dermatophytes should be considered important in evaluating the culture negative cases for dermatophytes as well as those cases ending up in treatment failure after empirical treatment for dermatophyte infections.
确定非皮肤癣菌性霉菌作为甲真菌病病原体的作用和模式。
病例系列。
2009年11月至2010年7月，拉瓦尔品第武装部队病理研究所微生物科。
从外观异常且甲真菌病治疗及检测失败的指甲获取指甲剪片和刮屑。采用显微镜检查（40%氢氧化钾涂片）以及在沙氏葡萄糖琼脂（SDA）、含氯霉素的SDA和含放线菌酮与氯霉素的SDA上进行培养来鉴定菌种。
在47例培养阳性病例中，有32例（68%）分离出非皮肤癣菌性霉菌。链格孢是最常见的菌种（46%）。仅从7例（15%）属于毛癣菌属的患者中分离出皮肤癣菌。8例（17%）分离出酵母菌。53%的病例未发现真菌生长。
在评估皮肤癣菌培养阴性病例以及经验性治疗皮肤癣菌感染后治疗失败的病例时，应将非皮肤癣菌视为重要因素。
