Fagundes Soares Garcia Anuska Conde, Marcon Alexandre Endres, Ferreira Douglisnilson de Morais, Barbosa dos Santos Esdras Adriano, do Amaral Viviane Souza, de Medeiros Sílvia Regina Batistuzzo
Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Av. Salgado Filho, s/n-Campus Universitário, Lagoa Nova, Natal, 59072-970, RN, Brazil.
J Environ Monit. 2011 Dec;13(12):3329-35. doi: 10.1039/c1em10582e. Epub 2011 Oct 21.
The present study evaluated the mutagenic potential of surface water from the Lucrecia dam. The Tradescantia-micronucleus (Trad-MCN) test and CBMN assay in human peripheral blood lymphocytes were applied, corresponding to an in vivo and in vitro system, respectively. Heavy metals and some physicochemical properties were also measured. Water samples were collected in November 2009 (dry season) and May 2010 (rainy season) at three different points. Results of both assays for raw water showed positive responses for the points analyzed when compared to the negative control. The CBMN assay showed that diluted water was still able to induce a significant increase in micronucleus frequency. For both assays, the highest mean MN was observed in the dry season. Chemical analyses detected an increase in heavy metal levels at the sampling points and in the different seasons. These findings indicate the presence of genotoxins, such as heavy metals, in the water, which may be affecting the entire ecosystem, as well as human health. More prolonged monitoring is recommended in order to better characterize this public water supply.
本研究评估了卢克雷西亚大坝地表水的致突变潜力。分别应用了紫露草微核（Trad-MCN）试验和人外周血淋巴细胞的CBMN试验，分别对应体内和体外系统。还测量了重金属和一些理化性质。2009年11月（旱季）和2010年5月（雨季）在三个不同地点采集了水样。与阴性对照相比，原水的两种试验结果在分析的点位均显示出阳性反应。CBMN试验表明，稀释后的水仍能显著增加微核频率。对于这两种试验，在旱季观察到最高的平均微核数。化学分析检测到采样点和不同季节的重金属含量增加。这些发现表明水中存在如重金属等基因毒素，这可能正在影响整个生态系统以及人类健康。建议进行更长期的监测，以便更好地描述这种公共供水情况。
