Biology Department, Uludag University, Faculty of Science and Arts, Gorukle, 16059 Bursa, Turkey.
Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2443-7. doi: 10.1016/j.fct.2010.06.007. Epub 2010 Jun 9.
In the present study, the in vivo micronucleus (MN) test in fish erythrocytes was used to evaluate the genotoxic potentials of water samples collected from four different sites along the Nilufer Stream which empties into the Marmara Sea on the north-west of Turkey. Nilufer Stream receives discharges of industrial and domestic wastes resulting from industrialization and urbanization activities in Bursa city. Nile tilapias (Oreochromis niloticus) were exposed to collected water samples under laboratory conditions for 3 and 6 days. Micronuclei analyses were carried out in peripheral blood erythrocytes. In addition to micronuclei, other nuclear abnormalities (NAs) such as bi-nucleated cells and binuclei with nucleoplasmic bridge and cells with blebbed, notched and lobbed nuclei, were assessed in the erythrocytes. Chemical analyses were also carried out in the water samples to assess the presence of major pollutants. MN and NA frequencies were significantly elevated in fishes exposed to water from polluted areas compared to those exposed to clean water sample. The results of this study indicate that Nilufer Stream is contaminated with potential genotoxic chemicals and the genotoxicity is possibly related with the industrial, agricultural and domestic activities.
在本研究中，采用鱼类红细胞体内微核（MN）试验来评估尼吕费尔河（Nilüfer Stream）四个不同地点水样的遗传毒性潜力。尼吕费尔河流经土耳其西北部的马尔马拉海，接纳了布尔萨市工业化和城市化活动产生的工业和生活废水。尼罗罗非鱼（Oreochromis niloticus）在实验室条件下分别暴露于采集的水样中 3 天和 6 天。在外周血红细胞中进行微核分析。除微核外，还评估了红细胞中的其他核异常（NA），如双核细胞、带有核质桥的双核细胞、泡状核、切迹核和分叶核细胞。水样中也进行了化学分析，以评估主要污染物的存在情况。与暴露于清洁水样的鱼类相比，暴露于污染水样的鱼类的 MN 和 NA 频率显著升高。本研究结果表明，尼吕费尔河受到潜在遗传毒性化学物质的污染，遗传毒性可能与工业、农业和家庭活动有关。
