Department of Surgery, Hsin-Chu General Hospital, Hsinchu, Taiwan.
Pancreas. 2012 Apr;41(3):447-54. doi: 10.1097/MPA.0b013e318229da41.
Grape-seed procyanidins (GSPs) can inhibit cell proliferation and invasiveness in various human cancers. However, the effect of GSP on pancreatic carcinoma cells has not been investigated.
Pancreatic carcinoma cell lines MIA PaCa-2 and BxPC-3 treated with GSP were assessed for viability by trypan blue exclusion, for cell cycle distribution by flow cytometry, for increased apoptosis by annexin V labeling, for their adhesion and invasion potential by evaluating their ability to penetrate through a matrix gel-coated Boyden chamber, and for changes in the levels of proteins involved in cellular events by immunoblotting.
Grape-seed procyanidin inhibited MIA PaCa-2 and BxPC-3 proliferation in a dose-dependent manner and induced G1-phase arrest of the cell cycle in BxPC-3 or mitochondria-mediated apoptosis in MIA PaCa-2. Grape-seed procyanidin also inhibited the adhesion and invasion potential of both cell lines in a dose-dependent manner, which are associated with the suppression of metalloproteases matrix metalloproteinase 9 or 2 (MMP-9 or -2) expression.
Grape-seed procyanidin inhibited the proliferation of pancreatic carcinoma cells by cell cycle blockage or apoptotic induction. The invasiveness was also suppressed by GSP through down-regulation of MMP-2 or MMP-9 in pancreatic carcinoma cells. Grape-seed procyanidin is a potential chemotherapeutic or preventive agent for pancreatic carcinoma.
葡萄籽原花青素（GSP）可抑制多种人类癌症中的细胞增殖和侵袭性。然而，GSP 对胰腺癌细胞的影响尚未得到研究。
用 GSP 处理胰腺癌细胞系 MIA PaCa-2 和 BxPC-3，通过台盼蓝排除法评估细胞活力，通过流式细胞术评估细胞周期分布，通过 Annexin V 标记评估凋亡增加，通过评估其穿透基质凝胶包被的 Boyden 室的能力评估其粘附和侵袭潜力，通过免疫印迹评估参与细胞事件的蛋白质水平的变化。
葡萄籽原花青素以剂量依赖性方式抑制 MIA PaCa-2 和 BxPC-3 的增殖，并诱导 BxPC-3 中的 G1 期细胞周期阻滞或 MIA PaCa-2 中的线粒体介导的细胞凋亡。葡萄籽原花青素还以剂量依赖性方式抑制两种细胞系的粘附和侵袭潜力，这与金属蛋白酶基质金属蛋白酶 9 或 2（MMP-9 或 -2）表达的抑制有关。
葡萄籽原花青素通过细胞周期阻滞或诱导凋亡抑制胰腺癌细胞的增殖。GSP 通过下调胰腺癌细胞中的 MMP-2 或 MMP-9 也抑制了侵袭性。葡萄籽原花青素是一种用于治疗胰腺癌的潜在化疗或预防药物。
