Wang Weihua, Zhan Leilei, Guo Dongqi, Xiang Yanju, Zhang Yu, Tian Muxing, Han Zhanjiang
Department of Food Science, College of Life Sciences, Tarim University, Alar, Xinjiang 843300, P.R. China.
Xinjiang Production and Construction Corps Key Laboratory of Deep Processing of Agricultural Products in South Xinjiang, Alar, Xinjiang 843300, P.R. China.
Oncol Lett. 2019 Feb;17(2):1741-1749. doi: 10.3892/ol.2018.9807. Epub 2018 Dec 6.
Grape seed proanthocyanidins (GSPs) have been demonstrated to exhibit potential chemotherapeutic efficacy against various cancer types. To determine the underlying molecular mechanisms involved in GSP-induced apoptosis, the present study prepared pancreatic cancer (PC) cells samples, S3, S12 and S24, which were treated with 20 µg/ml GSPs for 3, 12 and 24 h, respectively. Control cell samples, C3, C12 and C24, were also prepared. Using RNA-sequencing, transcriptome comparisons were performed, which identified 966, 3,543 and 4,944 differentially-expressed genes (DEGs) in S3 vs. C3, S12 vs. C12 and S24 vs. C24, respectively. Gene Ontology analysis of the DEGs, revealed that treatment with GSPs is associated with disruption of the cell cycle (CC) in PC cells. Additionally, disruption of transcription, DNA replication and DNA repair were associated with GSP-treatment in PC cells. Network analysis demonstrated that the common DEGs involved in the CC, transcription, DNA replication and DNA repair were integrated, and served essential roles in the control of CC progression in cancer cells. In summary, GSPs may exhibit a potential chemotherapeutic effect on PC cell proliferation.
葡萄籽原花青素（GSPs）已被证明对多种癌症类型具有潜在的化疗效果。为了确定GSP诱导细胞凋亡的潜在分子机制，本研究制备了胰腺癌（PC）细胞样本S3、S12和S24，分别用20μg/ml GSPs处理3、12和24小时。还制备了对照细胞样本C3、C12和C24。通过RNA测序进行转录组比较，分别在S3与C3、S12与C12以及S24与C24中鉴定出966、3543和4944个差异表达基因（DEGs）。对DEGs进行基因本体分析，结果显示GSPs处理与PC细胞的细胞周期（CC）紊乱有关。此外，转录、DNA复制和DNA修复的紊乱与PC细胞中的GSP处理有关。网络分析表明，参与CC、转录、DNA复制和DNA修复的常见DEGs相互整合，并在癌细胞CC进程的控制中发挥重要作用。总之，GSPs可能对PC细胞增殖具有潜在的化疗作用。
