Wang Weihua, Zhan Leilei, Guo Dongqi, Xiang Yanju, Tian Muxing, Zhang Yu, Wu Hong, Wei Yaxun, Ma Ganglong, Han Zhanjiang
College of Life Science, Tarim University, Alar, Xinjiang 843300, P.R. China.
Xinjiang Production and Construction Corps Key Laboratory of Deep Processing of Agricultural Products in South Xinjiang, Alar, Xinjiang 843300, P.R. China.
Oncol Lett. 2019 Mar;17(3):2777-2787. doi: 10.3892/ol.2019.9887. Epub 2019 Jan 4.
MicroRNAs (miRNAs) are small non-coding RNAs of 18-25 nucleotides that modulate gene expression at the post-transcriptional level. Grape seed proanthocyanidins (GSPs), which are biologically active components in grape seeds, have been demonstrated to exhibit anticancer effects. The current study investigated whether GSPs can regulate miRNA expression and the possible anticancer molecular mechanisms of GSPs. Pancreatic cancer (PC) cell samples, SS3, SS12 and SS24, were treated with 20 µg/ml GSPs for 3, 12 and 24 h, respectively. Control samples, SC3, SC12 and SC24, were also prepared. Using miRNA-seq, transcriptome analysis identified 24, 83 and 83 differentially expressed (DE) miRNAs in SS3 vs. SC3, SS12 vs. SC12 and SS24 vs. SC24, respectively. This indicated that treatment with GSPs could modulate the expression of miRNAs. Subsequently, 74, 598 and 1,204 target genes for the three sets of DE miRNAs were predicted. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that multiple target genes were associated with the proliferation and apoptosis of PC cells. In addition, a network was constructed of the DE miRNAs and the target genes associated with PC. The associations identified suggested that treatment with GSPs may inhibit the proliferation of PC cells through the modulation of miRNA expression.
微小RNA（miRNA）是一类长度为18 - 25个核苷酸的小型非编码RNA，可在转录后水平调节基因表达。葡萄籽原花青素（GSP）是葡萄籽中的生物活性成分，已被证明具有抗癌作用。本研究调查了GSP是否能调节miRNA表达以及GSP可能的抗癌分子机制。分别用20μg/ml GSP处理胰腺癌（PC）细胞样本SS3、SS12和SS24，处理时间分别为3小时、12小时和24小时。同时制备对照样本SC3、SC12和SC24。通过miRNA测序，转录组分析分别在SS3与SC3、SS12与SC12、SS24与SC24中鉴定出24个、83个和83个差异表达（DE）的miRNA。这表明GSP处理可调节miRNA的表达。随后，预测了三组DE miRNA的74个、598个和1204个靶基因。基因本体论和京都基因与基因组百科全书分析表明，多个靶基因与PC细胞的增殖和凋亡相关。此外，构建了DE miRNA与PC相关靶基因的网络。所确定的关联表明，GSP处理可能通过调节miRNA表达来抑制PC细胞的增殖。
