Department of Chemical and Biological Engineering, Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA.
J Biotechnol. 2012 Jan;157(1):159-66. doi: 10.1016/j.jbiotec.2011.10.001. Epub 2011 Oct 12.
Synthetic Escherichia coli consortia engineered for syntrophy demonstrated enhanced biomass productivity relative to monocultures. Binary consortia were designed to mimic a ubiquitous, naturally occurring ecological template of primary productivity supported by secondary consumption. The synthetic consortia replicated this evolution-proven strategy by combining a glucose positive E. coli strain, which served as the system's primary producer, with a glucose negative E. coli strain which consumed metabolic byproducts from the primary producer. The engineered consortia utilized strategic division of labor to simultaneously optimize multiple tasks enhancing overall culture performance. Consortial interactions resulted in the emergent property of enhanced system biomass productivity which was demonstrated with three distinct culturing systems: batch, chemostat and biofilm growth. Glucose-based biomass productivity increased by ∼15, 20 and 50% compared to appropriate monoculture controls for these three culturing systems, respectively. Interestingly, the consortial interactions also produced biofilms with predictable, self-assembling, laminated microstructures. This study establishes a metabolic engineering paradigm which can be easily adapted to existing E. coli based bioprocesses to improve productivity based on a robust ecological theme.
经工程改造用于共生的合成大肠杆菌群落相对于单培养物显示出更高的生物量生产力。二元群落的设计旨在模拟普遍存在的、由二次消耗支持的自然发生的初级生产力的生态模板。合成群落通过将葡萄糖阳性大肠杆菌菌株与葡萄糖阴性大肠杆菌菌株结合，复制了这种经过进化验证的策略，后者消耗初级生产者的代谢副产物。工程群落利用战略分工来同时优化多个任务，从而提高整体培养性能。群落相互作用产生了增强系统生物量生产力的突现特性，这在三种不同的培养系统中得到了证明：批式、恒化器和生物膜生长。与这三种培养系统的适当单培养物对照相比，基于葡萄糖的生物量生产力分别提高了约 15%、20%和 50%。有趣的是，群落相互作用还产生了具有可预测的、自组装的层状微观结构的生物膜。这项研究建立了一种代谢工程范例，可以很容易地适应现有的基于大肠杆菌的生物工艺，以基于稳健的生态主题提高生产力。
