Unidad de Investigacion en Enfermedades Oncologicas, Hospital Infantil de Mexico, Federico Gomez, Mexico City, Mexico.
Unidad de Investigación Medica en Oncologia, CMN sXXI IMSS, Mexico City, Mexico.
Chest. 2012 Apr;141(4):886-894. doi: 10.1378/chest.11-0633. Epub 2011 Oct 20.
B cells play an important role in allergic asthma. However, the mechanisms by which these cells are activated in the airways remain poorly understood.
We used a mouse model of ovalbumin (OVA)-induced allergic inflammation to study CXCL13 and to investigate the concentration of this chemokine in the BAL fluid derived from asthmatic and normal control subjects.
We found that OVA-challenged mice upregulate the CXCL13/CXCR5 axis, which is associated with several changes in their airways, including recruitment of B and CD4(+) cells, development of bronchial-associated lymphoid tissue, and airway inflammation. Treating sensitized mice with an anti-CXCL13 antibody reduced cell recruitment, bronchial-associated lymphoid tissue formation, and airways inflammation. Interestingly, measurements of CXCL13 using enzyme-linked immunosorbent assay showed that levels of this cytokine were significantly elevated in BAL fluid from subjects with asthma compared with control subjects (median, 162 [range, 120-296] vs 31 [range, 120-156] pg/mL; P = .005).
All together, these findings suggest that CXCL13 is involved in the allergic airway inflammatory process, and targeting this chemokine may constitute a novel approach in asthma.
B 细胞在过敏性哮喘中发挥重要作用。然而，这些细胞在气道中被激活的机制仍知之甚少。
我们使用卵清蛋白（OVA）诱导的过敏性炎症小鼠模型来研究 CXCL13，并研究哮喘和正常对照受试者的 BAL 液中这种趋化因子的浓度。
我们发现，OVA 挑战的小鼠上调了 CXCL13/CXCR5 轴，这与它们气道的几个变化有关，包括 B 和 CD4+细胞的募集、支气管相关淋巴组织的发育和气道炎症。用抗 CXCL13 抗体治疗致敏小鼠可减少细胞募集、支气管相关淋巴组织形成和气道炎症。有趣的是，酶联免疫吸附试验测量的 CXCL13 表明，与对照组相比，哮喘患者的 BAL 液中这种细胞因子的水平显著升高（中位数，162[范围，120-296]与 31[范围，120-156]pg/mL；P=0.005）。
综上所述，这些发现表明 CXCL13 参与了过敏性气道炎症过程，靶向这种趋化因子可能成为哮喘的一种新方法。
